The reconstructed oocytes proved that nuclei did envelope breakdown and the premature chromosomes condensed. On the fifth day, most embryos were diploid. The embryos were transferred to surrogate gilts, and three of them were found pregnant.One of them delivered a healthy transgenic piglet that did express the EGFP. Thereby, it was concluded that the combination of somatic cells by the replication defective vector with the nuclear transfer of colchicine treated donor does produce the transgenic pigs (Houdebine 19). In summary, the insertion of the DNA into the pronuclei of zygotes was a critical experiment that led to the research of transgenic pigs. The next stage was the transduction of the oocyte, which enables the integration of the defective retrovirus into the chromosomes. The process was applied to the pigs after a success in cattle.
Five pigs were used for the experiment; three were female and two male pigs. The effects of the transgene were same for both sexes. Transplant of organs was the main treatment aid aimed for with the usage of pigs. Body part tissues thereby are transferred into transgenic pigs. This is because transplant can be carried out between two species that are discordant such as the pigs and the human (Houdebine 30). This is because they have fewer genes that ate in common. Thereby so many pigs have been bred that are said to bear plenty of human flags in their cells. Other benefits of pigs are tat for years they have been used by human to treat diabetes. There insulin was known to cure diabetic diseases. Their heart valves were also used to replace those of human that were damaged.
Growth hormones were installed to the pigs to increase their growth rate and reduce the amount of feed that was required per unit of their weight gain (Houdebine 35). The most positive aspect with the hormone was that it ensured that