Reduction of ATP Hydrolysis Activity - Essay Example

These findings, together with comparing the crystal structures of T. thermophilus V1 with E. hirae V1, strongly suggest that the A subunit adopts a conformation in T. thermophilus V1 different from that in E. hirae V1. This fundamental difference results in ADP inhibition of T. thermophilus V1 by abolishing the binding affinity for phosphate during ATP hydrolysis (Hu, Wei-Shou, Zeng, & Berger 13).

An overlap of the PCR-based method constructed the preparation of Chimeric. The second procedure, on the other hand, involved the introduction of cysteine residues into the D subunit contained in the V1 (Hu, Wei-Shou, Zeng, & Berger 13). Lastly, the last portion of the experiment involved the introduction of protein concentrations of the already purified V1constructs which were established from the UV absorbance calibrated by the quantitative amino acid examination (Mather, 143).