...absence of N17Ras was shown to increase the number of invaded cells per field, then it is highly likely that invasion can be attributed to the presence of MMP-2, which is Ras-dependent. Again, as mentioned in part A, N17Ras competes with the wild-type Ras in the latter’s interaction with the factors that lead to the upregulation of MMP-2 (Kin et al. 55)
These findings were determined using gelatin zymogram assay, as well as in vitro invasion and transwell migration assays. Particularly, gelatin... A) According to Kin et al. (54), based on the results shown in a and b figures, Gα12/13-induced MMP-2 upregulation, invasion/migration needs Ras- andRac. How were the researchers able to arrive at such...