Got a tricky question? Receive an answer from students like you! Try us!

Critical evaluation of the use of indirect immunocytochemical techniques in diagnostic pathology - Essay Example

Only on StudentShare
High school
Author : hmarvin
Essay
Health Sciences & Medicine
Pages 4 (1004 words)

Summary

Immunocytological techniques as applied to immunohistochemistry (IHC) is an integral technique in present-day diagnostic pathology. This technique allows one to examine cytological details and tissue architecture better. …

Extract of sample
Critical evaluation of the use of indirect immunocytochemical techniques in diagnostic pathology

Immunocytological techniques as applied to immunohistochemistry (IHC) is an integral technique in present-day diagnostic pathology. This technique allows one to examine cytological details and tissue architecture better. Antigens introduced into the tissue will bind to labeled antibodies acting as specially designed reagents, and this technique allows one to assess the presence of specific antigen or protein. This binding event is visualized and examined under a microscope. Definition, Principles, and Development of IHC: IHC is a technique of antigen localization with antibody tagging and then tracking that antibody to detect the antigen. When an antibody is labeled by a specific technique, this can act like reagents in a designed antigen-antibody reaction. This reaction can be visualized by markers, such as, fluorescent dye, enzymatic reaction, radioactive tagging, or colloidal gold. These can detect cellular antigens in tissue sections. It started with discovery of immunofluorescence technique by Coons in 1941. Since then, techniques have developed to improve the sensitivity and specificity of this diagnostic tool, which basically detects antigen-antibody binding demonstrated with coloured histochemical reactions visible by light microscopy or fluorochromes with ultraviolet light. Later, techniques were developed to use enzymes like peroxidase and alkaline phosphatase or colloidal gold and the microscopy advanced to electron microscopy to make detections better ...
Download paper

Related Essays

CLINICAL CASE STUDY- Diagnostic Techniques in Pathology
The cough, she says started off as a common case. However, it got worse throughout the night a situation that forced her to seek medical attention from a General Practitioner. After pre-examination, the patient was asked to provide a sputum sample since hers was a productive cough. The sputum test results showed a characteristic yellow-green colour which formed part of the basis of her diagnosis. The patient was also found to produce chest sounds that pointed to a harsh rubbing and dull percussions notes on one side of her chest. Further tests for which she provided her blood sample and…
6 pages (1506 words)
Critical Evaluation of a Website
Also, it is possible to contact WWW@sil.org. There is no any information about updates and creation of this web page. It is possible to say that this document is a current one because the organization indicates a current year on the site. The site provides viewers with a link to the home page. Also, the graphics of this site and information display is appealing to users. The purpose of the page web is indicated on the home page. Thus, there is a need for up-to-date information and the exact date of all changes. The information discussed in the article is useful for my purposes because it…
2 pages (502 words)
Evaluation of Investment Appraisal Techniques
(Ross et al., 2002). The latter case is in a situation where only one project is being evaluated. In the event were the company is evaluating a number of projects, for which only one will be selected, the decision criteria is to first of all discard all projects with negative net present values and then select the project with the highest net present value among the positive NPV projects. (Ross et al., 2002; Myers and Brealey, 2002).…
12 pages (3012 words)
Critical evaluation
These two research methodologies, quantitative and qualitative, generally polarize along the objective (quantitative) and subjective (qualitative) continuum, yet Park and Ernst's study has aspects of both.…
10 pages (2510 words)
Capital Asset Management and techniques of its evaluation
Although the calculation is easy to understand and simple, it still has its limitations. It ignores the benefits, or the lack of, that occur after the payback period and more importantly, the method ignores the time value of money.…
4 pages (1004 words)
Critical Evaluation of Ipad
This is evident as the design of the Ipad software is very elementary and user-friendly. With the touch screen technology, users can use their fingers to access programs and files that they wish to access, just like a child playing with toys.…
8 pages (2008 words)
Clinicl Pathology
On the other hand, cells are the solid part of the blood. There are three types of cells present in the blood such as the red blood cells, white blood cells, and platelets.…
12 pages (3012 words)