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Genome Science and Biological Exploration - Essay Example

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Genome Science and Biological Exploration

The use of microarray technology to study gene-expressions in yeast S. cerevisiae first involves the construction of microarrays in four steps. The first of these steps involves generation of target DNA for spotting. The S. cerevisiae genome contains PCR products that significantly predict opening reading frames whose standard conditions and sequence-specific primers are essential in intensifying the reading frame and the intergenic sequencing of the S. cerevisiae (Hayward, et al., 2000). The second step involves the preparation of slides before engaging in carrying out the printing process. Importantly, these slides must be of high quality. Thereafter, printing the arrays takes place with the final step involving the processing of the microarrays after printing. In this step, the spotted DNA must be prevented from the nonspecific binding of probe DNA by using chemicals to block the unbound lysine on the surface of the slide (Hayward, et al., 2000). ...
 In line with this step, a microarray-referencing sample should be chosen in order to ensure that there is a collection of the adequate sample during the experimental procedure. Following the selection of the sample, the next step involves the isolation of RNA and probe generation. In this case, it involves lysing the frozen samples, centrifuging the emulsion obtained after incubation to remove any debris, and then proceeding with the extraction of the aqueous state. Finally, this process culminates in the precipitation of the total RNA that undergoes incubation, centrifugation, and resuspension to provide RNA that was pure in protein. After obtaining these samples of total RNA, the next step involves amplification of the total RNA to obtain double-stranded cDNA that undergoes hybridization and a sequence of three washes in order to increase the stringency (Wang, et al., 2000). Qn. 2 a) Importance of the yeast Saccharomyces cerevisiae as a model organism in the study of the cell cycle Model organisms are crucial in research since they provide researchers the opportunity to develop frameworks that facilitate standardized methods for analysis. The yeast S. cerevisiae as a model in the study of the cell cycle is important since the “cells lack the dense cortical actin cytoskeleton, which in mammalian cells gives structural support to the plasma membrane and maintains the cell shape” (Kaksonen, n.d, p.86). ...Show more

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In the essay “Genome Science and Biological Exploration” the author analyses the use of DNA microarrays, which reveals thousands of genes in action at a time. The use of microarray technology involves the construction of microarrays in four steps…
Genome Science and Biological Exploration essay example
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