These are bacteria's, they are one of the major groups of microorganisms together with the viruses, fungi, protozoa and algae.
To go on through this activity, materials are first was settled together with the medias and the counting performance and the methods to be done. The results were constructed in a summarized manner and are constructed with a form of graphical presentation and curves. Data gathered are correlated in a logical manner for generating a verified conclusion.
Bacteria as a microbe live in most of the world's habitats and are indispensable for normal balance life on earth. Bacterias so like as the other group of microorganism needs to live and require an environment suited to their needs for growth.
Bacteria's are unicellular and are lacking chlorophyll though they are plant-like. Different species of bacteria have different food requirements, which the environment must provide in terms of their nutritional needs. Since the bacterial nutrition is defined as the determination of the types and amounts of chemicals required for optimal growth of microorganism like
carbon for the heterotrophic; these are the bacteria which comprises the great majority of species, they must obtain their carbon and nitrogen from more or less complex organic compounds- carbon from proteins, fats and carbohydrates; nitrogen usually amino acids or nitrites.
autotrophies is carbon dioxide; they are the bacteria that grows in the absence of organic matter, they build their own food supply from simple inorganic substances, many of the soil bacteria is in this group, which utilizes carbon dioxide, ammonia or nitrites as sources of carbon and nitrogen,;
While the chemo tropic's source is chemicals; and the prototroph is the utilized light.
Microorganisms are quite versatile, because of their ability to adapt easily. And various nutrients play in the metabolism and growth of bacteria.
Materials and Methods
Microbial growth is measured by examining the increase in the population size of the bacteria whether direct or indirect. We count the number of cells using a counting grid and only living organism will be counted in the whole population on an agar medium. If we measure it indirectly, we measure the mass of cells whether dry or wet weight) or the turbidity of a liquid suspension with the use of spectrophotometer.
Here we have examined the influenced of temperature, like for instance in the growth of Escherichia coli and Staphylococcus epidermidis, they are measured in three (3) different temperatures for 26.5 hour. The spectrophotometer technique (indirect method) and viable count spread-plate technique (direct method) are used to monitor bacterial growth. . Microbiology as a science is very dependent on a number of specialized laboratory techniques. Laboratory steps routinely employed in experiments involving any microorganism such as bacteria are inoculation, incubation, isolation, inspection and identification.
Initially, a specimen must be collected from a source whether environmental or a patient.
1. Inoculation of a medium is the first step in obtaining a culture of microorganisms present
2. Incubation of the medium with microbes under the right conditions allows growth to visible colonies. Generally,