StudentShare solutions
Triangle menu

Enzyme Kinetics - Lab Report Example

Extract of sample
Enzyme Kinetics

The results were tabulated, statistically treated and interpreted to reveal that enzymatic activities are affected by changes in temperature, such that as the temperature of the environment where the chemical reaction takes place increase, the chances of the rate of molecular collision increases causing a consistent increase in the production of glucose. The same concept holds true to ph, substrate concentration, and enzyme concentration. Nevertheless, the rate of molecular reaction facilitated by enzymes ceases when enzymes as protein molecules are denatured because of extremes in these factors. But, a supplementary enzyme from an artificially concocted tablet like Lactaid when taken is capable of augmenting enzyme concentration in case the need arises.
Plants, animals, and microorganisms body growth and development are products of kinetics in metabolic pathways (Mader, 2001). The kinetics or the speeding-up, or the slowing down of chemical reactions in these metabolic pathways is caused by enzymes (Miller and Levine 2003). Enzymes are commonly known as protein molecules that are preoccupied with control of chemical reactions in the metabolic pathway. ...
Since enzymes are included in the process of metabolism, it is said that it also is responsible in producing all the chemicals in living organisms.
Additionally, it is also considered as important factor for the fast process of metabolism. But in every process there are different factors that enzymes are conditioned. These determinants greatly contributed for the dynamic process of a living organism's metabolism.
There were different laboratory studies conducted which determined that the speeding-up and slowing-down of metabolism are affected by its surroundings changes in temperature, intense shift in pH (high or low), which in turn affects the efficiency of enzymes. Another one is the substrate concentration, wherein this indicates the reaction of enzymes in a given condition, and lastly, the enzyme concentration which indicates the amount of enzymes in the substrates in a chemical reaction.
This laboratory study revealed the important role of enzymes to sustain life. It indicated the progress of ones' metabolism with the proper condition of enzymes. There are thousands of enzymes that are known and each of it has their particular work. In every living organism, the metabolism process if very different from one of each other. This will determine the metabolic enzymes that should be evident in for example, digestion.
There are thousands of enzymes that are evident and are important in every living organism. One of this is the enzyme lactase. Lactase enzyme is needed for proper digestion of lactose that is evident in dairy products. This typical enzyme is familiarly known to determine the cause and effect of improper digestion in living organisms.
As observed, majority of human adults are victims ...Show more

Summary

To test how temperature, ph, substrate concentration, and enzyme concentration affect hydrolysis of lactose was the interest of this research. This was done to learn the structure and function of an enzyme, to learn the relationship between substrates, enzymes, and products, to understand how different factors can affect the rate of an enzymatic reaction, to learn about lactose, lactase, and how to test for the presence of glucose with a Diastix, and to turn in a formal laboratory report…
Author : viviennebrown
Enzyme Kinetics essay example
Read Text Preview
Save Your Time for More Important Things
Let us write or edit the lab report on your topic
"Enzyme Kinetics"
with a personal 20% discount.
Grab the best paper

Related Essays

Enzyme Amylase Lab Report
It proved that high enzyme concentrations and optimum pH levels and temperatures increased enzyme activity. Introduction: Amylase is an enzyme that helps in breaking down starch to form sugars such as maltose and glucose. In human beings it is found in the saliva.
5 pages (1250 words) Lab Report
Enzyme Assays, Enzyme Kinetics
In general terms this was to introduce concepts related to enzyme kinetics. Introduction Enzymes are among several proteins generated in living cells to catalyze or accelerate the organism’s metabolic processes. Normally, enzymes are so selective and specific on the molecules they catalyze, referred to as substrate (Dixon, 1979).They normally do react specifically with a single substrate.
6 pages (1500 words) Lab Report
Kinetics Lab
3. Add 1 ml of starch indicator and 0.05 ml of 0.1 M EDTA solution from the Chemical Shelf to the same 250 ml beaker. This drop of EDTA solution is to minimize the effects of trace quantities of metal ion impurities that would cause spurious effects on the reaction.
5 pages (1250 words) Lab Report
Enzyme Kinetics Lab Report
understand how different factors can affect the rate of an enzymatic reaction, to learn about lactose, lactase, and how to test for the presence of glucose with a Diastix, and to turn in a formal laboratory report. The researcher utilized Lactaid which is an over the counter
14 pages (3500 words) Lab Report
Enzymology
Enzymology is a branch of science that studies the nature and functionality of enzymes. Catalase enzymes are susceptible to the environmental factors such as temperature, substrate concentration, pH level, enzyme concentration, and presence of enzyme inhibitors
5 pages (1250 words) Lab Report
The Effect of low pH on Enzyme Activity
The enzymes have specialized structures that work according to lock and key model. An enzyme is specific and cannot activate all kind of reactions, as a key can open certain lock only. Enzymes play a vital role
3 pages (750 words) Lab Report
Enzymes activity
Inhibitors also affect working of enzyme by competitively, non-competitively and mixed reaction. Method: a calibration graph that had been previously prepared was used. A Line-weaver Burk was plotted with reciprocal of substrate plotted against reciprocal of
7 pages (1750 words) Lab Report
Horseradish peroxidase assay
They only change the rate of reaction (Kovarik & Allbritton, 2011). The structures of enzymes comprise of a globular protein and a cofactor. The bonds holding the proteins in their structures are affected by temperature and pH changes. Such changes affect the
6 pages (1500 words) Lab Report
Enzyme lab
hrough the method of extracting the enzyme by blending it with potato tissue in phosphate buffer, the effects were analyzed on the effect of the dye guaiacol and the activity measured under different temperatures. The optimum temperature was obtained at 22.20C and above this
2 pages (500 words) Lab Report
Lactase Enzyme Activity
According to the author of the report, enzymes are capable of regulating the rate at which chemical reactions occur without being altered in the process. To be precise, enzymes achieve this function by lowering the activation energy necessary for the reaction to occur in cells.
6 pages (1500 words) Lab Report
Get a custom paper written
by a pro under your requirements!
Win a special DISCOUNT!
Put in your e-mail and click the button with your lucky finger
Your email
YOUR PRIZE:
Apply my DISCOUNT
Comments (0)
Rate this paper:
Thank you! Your comment has been sent and will be posted after moderation