StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Enzyme Kinetics - Lab Report Example

Cite this document
Summary
To test how temperature, ph, substrate concentration, and enzyme concentration affect hydrolysis of lactose was the interest of this research. This was done to learn the structure and function of an enzyme, to learn the relationship between substrates, enzymes, and products, to understand how different factors can affect the rate of an enzymatic reaction, to learn about lactose, lactase, and how to test for the presence of glucose with a Diastix, and to turn in a formal laboratory report…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER98.5% of users find it useful
Enzyme Kinetics
Read Text Preview

Extract of sample "Enzyme Kinetics"

Download file to see previous pages

The results were tabulated, statistically treated and interpreted to reveal that enzymatic activities are affected by changes in temperature, such that as the temperature of the environment where the chemical reaction takes place increase, the chances of the rate of molecular collision increases causing a consistent increase in the production of glucose. The same concept holds true to ph, substrate concentration, and enzyme concentration. Nevertheless, the rate of molecular reaction facilitated by enzymes ceases when enzymes as protein molecules are denatured because of extremes in these factors.

But, a supplementary enzyme from an artificially concocted tablet like Lactaid when taken is capable of augmenting enzyme concentration in case the need arises. Plants, animals, and microorganisms body growth and development are products of kinetics in metabolic pathways (Mader, 2001). The kinetics or the speeding-up, or the slowing down of chemical reactions in these metabolic pathways is caused by enzymes (Miller and Levine 2003). Enzymes are commonly known as protein molecules that are preoccupied with control of chemical reactions in the metabolic pathway. . Since enzymes are included in the process of metabolism, it is said that it also is responsible in producing all the chemicals in living organisms.

Additionally, it is also considered as important factor for the fast process of metabolism. But in every process there are different factors that enzymes are conditioned. These determinants greatly contributed for the dynamic process of a living organism's metabolism. There were different laboratory studies conducted which determined that the speeding-up and slowing-down of metabolism are affected by its surroundings changes in temperature, intense shift in pH (high or low), which in turn affects the efficiency of enzymes.

Another one is the substrate concentration, wherein this indicates the reaction of enzymes in a given condition, and lastly, the enzyme concentration which indicates the amount of enzymes in the substrates in a chemical reaction. This laboratory study revealed the important role of enzymes to sustain life. It indicated the progress of ones' metabolism with the proper condition of enzymes. There are thousands of enzymes that are known and each of it has their particular work. In every living organism, the metabolism process if very different from one of each other.

This will determine the metabolic enzymes that should be evident in for example, digestion. There are thousands of enzymes that are evident and are important in every living organism. One of this is the enzyme lactase. Lactase enzyme is needed for proper digestion of lactose that is evident in dairy products. This typical enzyme is familiarly known to determine the cause and effect of improper digestion in living organisms. As observed, majority of human adults are victims

...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Enzyme Kinetics Lab Report Example | Topics and Well Written Essays - 3500 words”, n.d.)
Retrieved from https://studentshare.org/miscellaneous/1502923-enzyme-kinetics
(Enzyme Kinetics Lab Report Example | Topics and Well Written Essays - 3500 Words)
https://studentshare.org/miscellaneous/1502923-enzyme-kinetics.
“Enzyme Kinetics Lab Report Example | Topics and Well Written Essays - 3500 Words”, n.d. https://studentshare.org/miscellaneous/1502923-enzyme-kinetics.
  • Cited: 0 times

CHECK THESE SAMPLES OF Enzyme Kinetics

Enzyme Assays and Enzyme Kinetics

hellip; In general terms this was to introduce concepts related to Enzyme Kinetics.... galactosidase enzyme Name: University: Course: Date: Abstract The main purpose of carrying out this experiment was to investigate and measure two parameters, Km and Vmax for ?... galactosidase enzyme.... Furthermore the PH change effect on the enzyme activity was investigated as well.... The substrate has the capacity of binding to the enzyme at a location referred to as active site (Di Cera, 1996)....
7 Pages (1750 words) Lab Report

Factors influencing the rate of enzyme catalysed reactions

Every enzyme is made up of a specific amino acid sequence (primary structure) and a resultant twisted polypeptide chain (secondary structure), which again folds (tertiary structure) to form structural cavities.... Role of enzyme: enzymes catalyze physiologic reactions by decreasing the activation energy level that the reactants (substrates) must reach for the reaction to occur.... he enzyme activity is determined by the rate of an enzyme catalyzed reaction under certain defined conditions2....
16 Pages (4000 words) Essay

The effect of enzyme concentration on enzyme activity

The enzymes remain… The rate at which the enzyme works is influenced by the following factors (users.... In the following experiment, the aim was to determine the effect on the rate of reaction by varying the quantity of the enzyme on the reaction process by measuring a physically/visually perceptible change in colour.... A milk protein, Casein was used which is subject to breakdown by the action of a protease enzyme.... The idea was to vary the concentration of the protease enzyme in a constant volume of the milk sample and measure the reaction time by the end point, taken as the time taken for the clearing up of the solution....
4 Pages (1000 words) Essay

Metabolic control analysis

The model aids in understanding the control that each enzyme wields on the fluxes and Metabolic control analysis The purpose of metabolic control analysis and how it relates to Enzyme Kinetics Metabolic control analysis is a model that is used to examine the distribution of fluxes, and other metabolic concentrations in the metabolic pathways among the different enzymes found within the pathway (Heinstra & Geer, 1991).... Enzyme Kinetics is the study of the way enzymes catalyze chemical reactions....
2 Pages (500 words) Essay

Enzyme Kinetics of Inhibition

The reaction is usually expressed as In Enzyme Kinetics, the Michaelis Menten reaction relates the reaction rate (ν) to [S] which is the concentration of a substrate.... enzyme activity is affected by temperature, pH and… Inhibitors also affect working of enzyme by competitively, non-competitively and mixed reaction. Method: a calibration graph that had been previously prepared was used.... The enzymes control the reactions by ensuring that every reaction is catalyzed by a specific enzyme and at a particular point in a cell....
7 Pages (1750 words) Lab Report

The Effect of Increasing Concentration of Enzyme

The experiments very well demonstrated the saturation kinetics of the increasing enzyme.... The paper 'The Effect of Increasing Concentration of enzyme' presents Rennin which was chosen as the enzyme and milk as its substrate since rennin is responsible for the curdling of milk in infants.... The velocity of a chemical or enzyme reaction is almost doubled for every 10o C increase in temperature because the increase in temperature enhances the kinetic energy of the substrates and thus increases the proportion of substrates reaching the transition state (Kennel and Rodwell 2006)....
10 Pages (2500 words) Lab Report

Analyzing Protein

For Enzyme Kinetics, Spectrophotometer was blanked and the contents of 50 micromol guaiacol tube added to tubes of diluted enzymes before reading the absorbance levels and calculating the initial velocity of each substrate concentration.... This work called "Analyzing Protein" describes the effects of enzyme concentration and ph on peroxidase enzyme activity.... The author takes into account that peroxidase enzyme is specific to pH 4 while enzyme reaction is directly proportional to concentration optimal concentration ensures that the rate of reaction is high and steady for a long period of time....
8 Pages (2000 words) Research Paper

Effect of Enzyme Concentration, Temperature and pH on Enzymes

This lab report "Effect of enzyme Concentration, Temperature and pH on Enzymes" discusses enzyme as a biological molecule that speeds up biochemical reactions.... hellip; The specificity of an enzyme is determined by characteristics of the R-groups of the amino acid residues that form the active site of an enzyme.... An apoenzyme is an inactive enzyme and the active enzyme including the cofactor is referred to as a holoenzyme....
6 Pages (1500 words) Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us