Got a tricky question? Receive an answer from students like you! Try us!

Immunolocalization of the microtubule cytoskeleton - Essay Example

Only on StudentShare
College
Essay
Miscellaneous
Pages 5 (1255 words)

Summary

Proteins are probably the most diverse substances found in the cells found in both the single and multicellular organisms. Estimated around 10 000 different proteins, each protein has its specific function. Cellular functions are often localized in specific compartments of the cell, and being able to localize the unknown proteins may provide important information in determining the function of the proteins…

Extract of sample
Immunolocalization of the microtubule cytoskeleton

The most prominent methods are: Western blot, spectrophotometry, enzyme assay, immunoprecipitation and immunostaining. In immunostaining, the method used during this procedure, an antibody is used to detect a specific protein epitope. These antibodies can be monoclonal or polyclonal. Then enzymes such as horseradish peroxidase or alkaline phosphatase are commonly used to catalyse reactions that give a coloured or chemiluminescent product. Fluorescent molecules can be visualised using fluoresence microscopy.
During this experiment, the distribution of the protein tubulin in normal rat kidney (NRK) cells is explored. A monoclonal antibody that is specific for the -subunit of tubulin is used. Tubulin polymerizes into long, 25-nm wide microtubules that we will visualize with tubulin antibodies. The formation and maintenance of microtubules is cold sensitive. At 4C, microtubules are destabilized and they depolymerize. At 37C, human body temperature, they remain polymerized. Photograph 1 shows cells incubated at 4 0C, while photograph 2 shows cells incubated at 37 0C. At this stage of the procedure, microtubules cannot be detected in either of the photographs.
3 separate plates are used to create the NRK cells culture. ...
Download paper
Not exactly what you need?

Related Essays

Biology High School Essay
For example, a cell in a hypotonic solution will swell, while a cell in a hypertonic solution will shrink.…
2 pages (502 words)
The Essential Features of Prokaryotic Replication
This is nothing but duplication of the nuclear material contained in the nucleus of the cell, be it pro or eukaryotes. Replication is an essential phenomenon related to growth and division of the cell. Growth and division refer not simply to the accretion of biomolecules, but to the replication of an integrated pattern of functions and structures. The continuity of cellular structure is a necessary complement to the continuity of genetic information. This occurs through the process of replication (Burton and Engelkirk, 2001, 134-150).…
4 pages (1004 words)
Cell Structure
Plant and animal cells each have a true nucleus because they are both eukaryotic organisms. This central membrane-bound compartment is the site of storage for most of the cell's genetic material. Within the nucleus there is a nucleolus which produces ribosomes. Likewise, both plant and animal cells have corresponding organelle structures with identical functions. This includes: rough endoplasmic reticulum, smooth endoplasmic reticulum, peroxisomes, lysosomes, Golgi bodies, secretory vesicles, mitochondria, microtubules and microfilaments, cell membrane, cytosol, and a centrosome.…
2 pages (502 words)
'Discuss the biology of Alzheimer's disease'
The clinical features of Alzheimer's disease (AD) are, loss of short-term memory, deficient in praxis (ability to perform skilled movements) and the skill of reasoning and judgment (Doraiswamy PM, 1997). These symptoms arise from involvement of the temporal lobe, hippocampus, and the parietal association cortices, with lesser involvement of frontal lobes, until the disease is in its later stage. A second most prominent neuropathological feature which is also present in AD is the complex, fibrilar deposits in the cortex of the brain; this is known as senile and amyloid plaques. Amyloidal…
3 pages (753 words)
A functional DyneinMicrotubule Network Is Required for NGF signaling through the Rap1MAPK pathway
Neurons are known to be the receptors and conveyors of signals throughout our body.One of the processes that have gotten much attention is the mechanism which governs the transport of neurotrophin to the neurons. NTs have been proven to be essential to the survival and proper function of the neurons by the works of many studies such as that of Campenot and MacInnis (2004) which have identified that the absence of NTs results to destruction of the cell body.Establishing the importance of NTs on neurons was one thing; finding out how it is transported to the cell body was another. Shedding light…
3 pages (753 words)
Microtubules
Microtubules …
6 pages (1506 words)