StudentShare solutions
Triangle menu

Clinical and Laboratory Diagnosis of Salmonellosis - Essay Example

Extract of sample
Clinical and Laboratory Diagnosis of Salmonellosis

Laboratory Manual and Workbook in Microbiology Applications to Patient Care).
It is also called as bacillary dysentery. Universally gastro-enteritis is caused by Shigella. It may cause bloody diarrhea also called dysentery or cause non bloody diarrhea. Shigella gains entry through epithelial lining of large intestine and obliterate the intestinal mucosa. The infection is highly contagious and is responsible for over 600,000 or more deaths per year. Most victims are from developing nations and in areas of overcrowding where poor sanitation persist; jails, mental hospitals, refugee camps, daycare or in primary schools (Scarpignato, C, Lanas A, Bacterial Flora in Digestive Disease: Focus on Rifaximin (Digestion)).
Classical symptoms include watery loose stool, abdominal pain, mild fever, abdominal cramps, painful stools, frequent stools with bloody mucus. Some of the strains of Shigella are known to produce toxin that result in "hemolytic uremic syndrome"( Scarpignato, C, Lanas A, Bacterial Flora in Digestive Disease: Focus on Rifaximin (Digestion)).
Clinical Sample: Stool/ feces/ blood/ rectal swab (Josephine A. Laboratory Manual and Workbook in Microbiology Applications to Patient Care)Positive cultures are obtained from blood-tinged plugs of mucus of freshly passed stools. Rectal swabs are collected if arrangements are there for rapid processing of the sample or holding solution containing: buffered glycerol saline is available (Scarpignato, C, Bacterial Flora in Digestive Disease: Focus on Rifaximin (Digestion)).
Microscopic Examination: Bacillary dysentery characterized by sheets of PMN
Morphology & Staining: Differential Gram staining is performed show gram negative bacilli.
Processing of the clinical sample is done with the following protocol to confirm causative agent.
(Josephine A. Laboratory Manual and Workbook in Microbiology Applications to Patient Care)

Media: Low selectivity: MacConkey, EMB
Intermediate Selectivity: Xylose Lysine Desoxycholate, Desoxycholate Citrate Agar, Salmonella Shigella agar and Hektoen enteric agar (HE)
Highly selective: Bismuth sulphite (BS) agar and brilliant green agar (BG).
Biochemical Tests:
Serological Tests of Salmonellosis: Widal test (H and O agglutination for typhoid and paratyphoid patients), CIEP, Haemagglutination, ELISA, Bactericidal Antibody test, Adherence test for detection of IgM antibodies, RIA, Co-agglutination test, Latex agglutination test, PCR, Diazo test of Urine, bacteriophage typing (Josephine A. Laboratory Manual and Workbook in Microbiology Applications to Patient Care).
For detection of Salmonella, 8 hour of pre-enrichment is performed, persuaded by automated DNA extraction and a sensitive real-time PCR. Optimization of this method is done to obtain highest possible yield of cells and DNA to ensuring public health (Josefsen, M. H., Appl. Environ. Microbiol. 2007).
Serological Tests of Shigellosis: Slide agglutination with antisera for serogroup and serotype, PCR, ELISA, Monoclonal Antibodies test.
Biochemical reactions: MR +, reduce nitrate to nitrite, citrate utilization-ve, inhibited by KCN, H2S-ve,catalase+ve, oxidase+ve, ...Show more


Clinical features of Salmonellosis or gastroenteritis encompass physical examination and medical history, this comprise current medications, recently consumed food items, recent travels, restaurants visits. The nature of the food consumed viz. contaminated eggs/ poultry/ unpasteurized milk or juice, cheese or contaminated raw fruits and vegetables or consumption of water contaminated with fecal matter (Josephine A…
Author : terrill61
Clinical and Laboratory Diagnosis of Salmonellosis essay example
Read Text Preview
Save Your Time for More Important Things
Let us write or edit the essay on your topic
"Clinical and Laboratory Diagnosis of Salmonellosis"
with a personal 20% discount.
Grab the best paper

Check these samples - they also fit your topic

Virtual Laboratory
Many biotechnological experiments can be easily performed and explained using virtual laboratories in an effective manner. With the arrival of internet and computer technology in modern education, limitations like distance and costly instruments are no more a hurdle.
3 pages (750 words) Essay
Laboratory Medecine
(This line is important to show that you have some knowledge of the field - it cannot get simpler) In simpler words, the duties and responsibilities of a medical laboratory technician revolve around the collection and categorization of blood samples into types and groups.
3 pages (750 words) Essay
Laboratory Report
What was the latency of the response Answer: The data from sheet 1 showed the elbow flexion unloading response of the biceps. The weight was removed unexpectedly as we can see the unloading reflex in the bicep provoked by the load release. The latency time is about 55 milliseconds.
2 pages (500 words) Essay
Evidenced Based Practice (EBP) in Medical Technology in private clinical laboratory setting
These clinical laboratory that are conducted to assist in patient care amount to seven billion tests every year. (Mariani, M.S., 2003). Private clinical laboratories provide large number of
5 pages (1250 words) Essay
Rewrite my project
They respectively cause the diseases, campylobacteriosis, salmonellosis, and shigellosis. These have very similar symptoms, are infectious, and have the potential to reach
3 pages (750 words) Essay
Discuss the role of the laboratory in the diagnosis, classification and monitoring of the Myelodysplastic syndromes
The condition can involve any of the lineages of the myeloid system, including megakaryotic, granulocytic and erythrocytic hematopoiesis and is considered to be a premalignant condition with the potential to progress to acute myeloid
6 pages (1500 words) Essay
The laboratory diagnosis, monitoring and management of the diabetic patient
In addition, Diabetes is caused either by lack of insulin or because of the availability of factors that oppose the functionality of insulin.
9 pages (2250 words) Essay
What is formulation, as used in clinical psychology practice, and how does it differ from diagnosis
Medical diagnosis on the other hand refers to a doctor’s attempt to determine a disease in a patient thereby recommend a treatment. Diagnostic procedure includes an extensive description of the feelings and
4 pages (1000 words) Essay
Cultural Diversity in clinical laboratory sciences
Among the Asians, mystical healing is evidenced by practices such as rubbing a coin on the body of a sick person. Coin rubbing is a popular traditional healing process. Elders in both
2 pages (500 words) Essay
Medical Ethics in the clinical laboratory science
However, he informs me of the job he had applied for, and they ask for a reference report regarding his performance. After learning about the new job roles,
2 pages (500 words) Essay
Hire a pro to write
a paper under your requirements!
Win a special DISCOUNT!
Put in your e-mail and click the button with your lucky finger
Your email
Comments (0)
Click to create a comment
Contact Us