fluorophore through the absorption of light energy, a transient excited lifetime with some
loss of energy, and return of the fluorophore to its ground state accompanied by the
emission of light. Due to the energy lost during the transient excited lifetime, the light
energy emitted is always of a longer wavelength than the light energy absorbed, and that
is used to study different life processes (Molecular expressions).
Today, there is an increased use of these techniques encouraged mainly by labeled
antibody techniques (Coons and Kaplan, 1950) and by application of fluorescent dyes as
tracers in histochemical techniques. Aminoacridine compounds have special affinity for
nucleic acids; a sensitive fluorescence technique in which acridine orange is used for the
identification of DNA and RNA in mammalian cells (Anderson, Armstrong, and Niven,
1959). Thus using fluorescence techniques and microscopy, the precise location and
dynamics of intracellular components labeled with specific fluorophore designed for the
cell system and the targeted interaction as applied to a pharmaceutical agent. This domain
also, as a result, includes the study of other physicochemical properties of the concerned
molecule, diffusion coefficient, transport characteristics, and above all the interaction
with other biomolecules present. When applied to the field of study of pharmaceuticals
and their effect on cell systems, this can allow one to study the phenomenal response in
fluorescence to localized cellular environmental variables, such as, variation in pH,
viscosity, refractive index, ionic concentrations, membrane potentials, and solvent
polarity in living cell systems and tissue preparations with extraordinarysensitivity...
Anderson, E. S., Armstrong, J. A., And Niven, J. S. F., 1959. 'Observation Of Virus GrowthsWith Aminoacridines.' 9th Symposium Of The Society For General Microbiology, April,1959. Cambridge (University Press).
Medical Research Service, Department Of Veterans Affairs Medical Center,1 And Oregon Health And Science University,2 Portland, Oregon, And Department Of Biochemistry, Mahidol University,Bangkok, Thailand3