Write 020 and your name on another piece of filter paper. Follow steps 3-5 to transfer this volume of liquid to the filter paper. What volume in l does 020 represent
8. Now reset the numbers in the window to read 024 (fig.). Write 024 and your name on another piece of filter paper and follow steps 3-6. What volume in l does 024 represent
By this experiment we measured very small volumes of liquids and gels like DNA. Proper pipetting and sterile technique is essential for correct result. If there is any inaccuracy in pipetting or in sterile technique then it may cause poor and incorrect results. By using sterile technique we developed an ideal environment which protects our sample from contamination. If we put our finger on the tip of the pointed end then sample becomes contaminate.
Many laboratory experiments based on the microbiology or on the micro chemical protocols and small volume of DNA has to measure with pure cultures which is done with the help of two techniques-Micropipetting and sterile pipetting. These techniques take us towards nearer to the correct results. Use of these techniques is very important for the better result.
We can obtain ideal environment by this method 'Sterile Technique'. This helps us to gain better results. It means that in this process sterile surfaces or sterile media is protected from contamination by