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Northern (RNA) Blotting Technique
Pages 1 (251 words)
The northern (RNA) blotting technique, a method for investigating RNA molecules, is extensively used to compare the amounts of a specific mRNA in cells (Lodish et al, 192) and offers approximation of gene transcript size in kilobases. (Allison, 249) To analyze the albumin-encoding mRNAs with DNA probe, the unbroken mRNA molecules, purified from mutant and control liver cells, are separated into many bands by gel electrophoresis.
When the film is developed one finds "bands only in those lanes containing a tissue where the gene represented by the probe has been expressed." (Hartwell at al., 395) Therefore, liver cells albumin RNA of normal amounts and normal size are made from the mutant mice and whichh is identified in greatly reduced amounts. (Manipulating proteins, DNA, and RNA, 498)
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