College
Lab Report
Miscellaneous
Pages 4 (1004 words)
Download 0
DNA is the genetic material responsible for the development of all forms of life. It is found in the nucleus, mitochondria and chloroplast (in plant cells). In nucleus, DNA is associated with the histone proteins and is linear in shape whereas in organelles like mitochondria and chloroplasts, DNA is circular and is not associated with histone protein…

Introduction

One purine pair with one Pyrimidine with hydrogen bond to make the double stranded DNA. Adenine (A) pairs with Thymine (T) with double H-bond and Guanine (G) pairs with Cytosine (C) through triple H-bond.
Isolation procedure requires disruption of cells so that the cell content comes out, followed by sedimentation of the cellular debris on application of centrifugal force and to collect the DNA from the supernatant. These DNA fragments are separated using gel electrophoresis. The process encompasses separation, based on their size, the pore size of the gel, the voltage gradient applied and the salt concentration of the buffer. Larger pore size is for the separation of fragments larger than 500- 1000 bp and smaller pore of agarose gels are used to resolve fragments smaller than 1000 bps and can be visualized. The technique of electrophoresis is based on the fact that since DNA contains phosphate group, it is negatively charged at the neutral pH. When electric potential is applied, it moves towards the positive terminal.
The solidified agarose gel is inserted into the electrophoresis chamber and is just covered with buffer. The DNA sample is mixed with the loading buffer and then pipette in the sample wells. ...
Download paper
Not exactly what you need?

Related papers

Mitochondrial DNA
This is accomplished in humans by the sequencing one or more of the hypervariable control regions (HVR1 or HVR2) of the mitochondrial DNA (Schwartz & Vissing 2002).…
3 pages (753 words)
Forensic Science: DNA Evidence
DNA identification may allow sparing the world from personality violent crimes. If the database would be created that would contain the genetic samples of every dweller of our planet, the offender would be found in couple of hours or even minutes after the crime "Any type of organism can be identified by examination of DNA sequences unique to that species". (Human Genome Project Information,…
5 pages (1255 words)
Rapid Colony Transformation of E -Coli with Plasmid DNA
Recovery: LB broth is added to the DNA/cell suspension and incubated at 37C before being put on plates with different selected antibiotic resistant markers. Transformed cells recover from the treatment, amplify the transformed plasmid and begin to express the antibiotic resistant strain.…
3 pages (753 words)
DNA Fingerprinting
Jeffreys began at the simplest, by detecting single copies of the genes and the phenomena of introns. It was this research that introduced the genuine concepts of restricted fragment length polymorphism or RFLP.(Newton, 2004, np) By this technique it was possible to divide the DNA in to smaller pieces, which in turn led to discovery of SNP or single nucleotide polymorphism. The SNP became a…
8 pages (2008 words)
Unraveling the mystery of DNA
Whenever we think of the word DNA our mind wanders to scientists frowning at a microscope and the likes of the cast of "X-files" or some team in the Discovery channel launching a Criminal investigation. Interestingly the concept of DNA analysis has helped many fields of criminal investigation and genetic engineering. The "chemistry" of the "DNA" has become an integral part of biology and physics…
8 pages (2008 words)
DNA Sampling
The police, because of legal concerns, refuse to pursue this lead by acquiring samples from each of the thousands of persons of interest to it. The investigators' second-best lead was the footage shot at a truck stop on the night Falconio vanished. Bradley Murdoch was interviewed because his appearance and match with truck video, and passed over for DNA sampling. But Murdoch provided an apparent…
7 pages (1757 words)