It first reduces imbibition of water because of the lowered osmotic potential of the medium (Bliss et al., 1985). Second, it causes toxicity; that is, it changes enzymatic activity, hampers protein metabolism, upsets plant growth regulators balance, and reduces the utilization of seed reserves. It may elicit changes at ultrastructural, cellular and tissue, or even at organ levels (Dell’Aquila & Spada, 1993). Salinity interacts with certain plant and environmental factors during germination. Among these plant factors are seed coat, dormancy, seed age, seed polymorphism, and seedling vigor. Environmental factors include temperature, light, availability of water, and oxygen (Pessarakli, 1999).
In this experiment, it will be determined if salt will interfere with the rate of germination of soybean seeds. To assure that all other factors are held constant, a standard germination test will be undertaken under optimum conditions for seed germination.
2. Five rectangular plates (12”x5”) will be lined with paper towels pre-moistened by different solutions. Plate A is the control and it will be moistened with distilled water. Plate B will be moistened by the 0.5M NaCl solution, Plate C will be moistened with 1M NaCl solution, Plate D will be moistened with 2M NaCl solution and Plate E is moistened with 3M NaCl solution.
4. In the next three days, the paper towels in the plates will be moistened twice a day (10 AM and 4 PM) with the appropriate salt solution to prevent water loss due to evaporation. Also, the germinated seeds in each plate will be counted and recorded. . For this experiment, we will define germination as the breaking of the seed coat and the emergence of the radicle.
With the obtained results, the Chi-Square Test will be used because it will be compared how well an observed breakdown of germinated seeds over various categories fits some expected breakdown, such as an equal breakdown of