In this paper, bactericidal actions of silver ion solutions on Escherichia coli and Staphyloccocus aureus, as model organisms have been used. The main objective of this study is to find out whether silver ions can kill…
Incubate at 37C in a shaker incubator for overnight culture so that the bacteria would be in log phage. After that spin cultures at 6000 PRM for 10 minutes. Remove supernatant, leaving 5 ml in each tube. Resuspend pellet in the remaining supernatant and add 1ml of sterile nutrient broth to disposable plastic curvette and blank spectrophotometer. Add 1ml E.coli culture to plastic curvette and record A600 (make sure A600 ˃ 0.5). For each Ag+ compound (WI, WII, WIII, WIV, and WV) and +Ve control, take 10 ml dilution. Prepare 17 autoclave from liquid culture tubes from nutrient broth. The final volume is 10 ml.
Add 50 ml of E. coli for each different compound of Ag+ silver ions. In the same way, add 50 ml of S. aureus for each different compound of Ag+ silver ions. For each compound of silver ion one needs to incubate at 370 C in zero time, 4 time and 24 time to try to get a Time serious at the effect concentration to take samples at 0 hour, 4 hour and 24 hour to try get a Time: Concentration for Lethal does. At zero time for small tubes for each compound of silver ions and control, make dilution at 1:1, 1:102, 1:103 Spread plate. 50 μl of circled dilutions above on nutrient agar plates. Spread 50 μl of – ve control (no bug) controls. Incubate over night culture at 37 C.
At 4 times for small tubes of each compound of silver ions and control, prepare before taking sample from them and make dilution at 1:1, 1:102, 1:103. Spread plate. 50 μl of circled dilutions above on nutrient agar plates. Spread 50 μl of – ve control (no bug) controls. Incubate over night culture at 37 C.
At 24 time for small tubes of each compound of silver ions and control what I prepared before take sample from them and make dilution at: 102, 1:103, 1:104, 1:105, 1:106. Spread plate. 50 μl of circled dilutions above on nutrient agar plates. Spread 50 μl of – ve control (no bug) controls. Incubate over night culture at 37 ...
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