Spurious results may be obtained in some cases that departs from the expected norms for a particular species, may be attributed to strain differences within the given species. The unknown samples are required to be handled with care and precision. Identification procedures adopted, identified four bacterial species in the unknown samples, they are: Enterococcus faecalis, Citrobacter freundii, Klebsiella pneumoniae and Streptococcus pyogenes
Microorganisms are ubiquitous and they provide benefit or show their harmful impact on the human/ animal or plant population in varied ways. The body of knowledge that has accrued since these early years has been instrumental in making clinical microbiology a major component of laboratory or diagnostic medicine. It is essential to have an understanding towards these microorganisms for this isolation and identification of these infectious pathogens is imperative. This understanding aids in rapid diagnosis and treatment of the disease, prudently, intelligently and rapidly.
Many experiments are being carried out that have relevance with their application in the clinical microbiology. These experiments encompass, isolation and identification of unknown cultures, the use of selective and differential media and various biochemical tests used to separate and identify various microorganisms (Messeley, 2003).
The first step to onset the experiment is to perform Gram staining procedure. This segregates the gram positive and gram-negative organisms. This is followed by the streak-plating to observe the colony characteristics. The next step is the use of selective media for the preparation of pure cultures followed by the performance of biochemical tests. The present study was performed to apply all the methodologies learnt in the microbiology laboratory class designed for the detection of an unknown bacterium (Messeley, 2003).
Prepare Nutrient agar broth subculture of unknown sample and refrigerate