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Introduction of crude oil biodegrading by some marine bacteria and reduce sulphur component - Book Report/Review Example

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The role of microorganisms in degradation of marine oil spills is being constantly investigated as the fate of petroleum compounds in marine environment depends on their transformation or degradation by microbes. More than hundred known species of bacteria and fungi play a role…
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Introduction of crude oil biodegrading by some marine bacteria and reduce sulphur component
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Introduction of crude oil biodegrading by some marine bacteria and reduce sulphur component

n of marine oil is time-consuming as a result extensive damage can occur to the marine organisms and indirectly upon humans when they consume such organisms. This time lag is effectively being reduced through processes such as bioremediation through which the available bacterial population is altered or increased by addition of exogenous microbes and stimulation of indigenous population (Bioremediation methods for Oil Spills, n.d). Processes such as eutrophication have lead to increased production of organic matter which in turn results in anaerobic conditions due to oxygen uptake by heterotrophic organisms. Under such conditions mineralization of organic matter by processes such as sulphate reduction by marine-sulphate reducing bacteria can play a vital role. These anaerobic bacteria utilize sulphate as the final electron acceptor during their respiration which releases hydrogen sulphide into the environment. A number of enzymes are involved in this process and the large amounts of hydrogen sulphide released are vital from an ecological and economic viewpoint. Studies have also revealed a direct relationship between the number sulphate reducing bacteria and the amount of hydrogen sulphide released (Mudryk, Podgórska & Bolalek, 2000). The identification of microbes which degrade crude oil has been done by serial dilution of sea water samples and plating them on mineral media followed by spraying a layer of crude oil on the agar plate. The bacterial isolates which appear after an incubation period are then screened by using overlay techniques (Sakalle & Rajkumar, 2009). In another techniques beach simulation tanks oil-polluted cobble stones collected from a contaminated beach were put into tanks which were filled with sea water. The gravel was then inoculated into enrichment cultures followed by broth and agar culture (Cerniglia & Perry, 2007). The DNA was then isolated from pure cultures and nucleotide sequencing of 16S rDNA was carried out which was then used ... Read More
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