We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Nobody downloaded yet

Protien refording - Essay Example

Comments (0) Cite this document
Summary
Biochemical research intrinsically involves proteins, and biochemists seek to understand the nature of many characteristics of proteins, including protein folding. As the field grows, many researchers are using protein folding as critical steps in biotechnological experiments…
Download paper
GRAB THE BEST PAPER

Extract of sample
Protien refording

During initial production and isolation of proteins, many factors such as overproduction, solvent interactions, mechanical interference, or others may result in the denaturation of proteins.
Understanding protein folding involves understanding both the energy landscape of the protein system, and refolding techniques have been significantly improve as time-resolved techniques, including neutron scattering, have been developed and perfected by researchers around the globe. The techniques involve observation of protein dynamics in order to assess the critical point of refolding, information which can lead to the development of refolding solutions (Bu et al. 2001). Neutron scattering and similar inventive techniques, such as stopped-flow florescence.
In order to scale up for commercial use, protein refolding techniques must be scale invariant, compatible for a large range of proteins, simple to automated, and overall economical. Methods that rely on denaturant dilution and column-based methodology generally will meet these criteria (Middelberg 2002). The technology of refolding has grown exponentially in the past decade, and new methods must be carefully designed to facilitate the automated and rapid determination of the conditions that must be met for refolding in order to be commercially viable. It, however, remains to be seen if researchers can translate new technologies—and possibly even the discovery of a new protein state—into technology that will improve efficiency in bimolecular research industries.
Before use, proteins are generally solubilised before use in high concentrations of quanidinium chloride (GdmCl) and urea (De Bernardez 1998 and Schwarz et al. 1998). Either of these two solvents may cause certain proteins to denature, and refolding involves diluting to a low concentration zone. ... Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Protien refording Essay Example | Topics and Well Written Essays - 2500 words”, n.d.)
Retrieved from https://studentshare.net/miscellaneous/406268-protien-refording
(Protien Refording Essay Example | Topics and Well Written Essays - 2500 Words)
https://studentshare.net/miscellaneous/406268-protien-refording.
“Protien Refording Essay Example | Topics and Well Written Essays - 2500 Words”, n.d. https://studentshare.net/miscellaneous/406268-protien-refording.
  • Cited: 0 times
Comments (0)
Click to create a comment
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Method of validation and comparison between the sysmex CA1500 and sysmex CS2100i
To date, worldwide laboratories use coagulometers or automated analyzers to analyze screening coagulogram, including Activated Partial Thromboplastin Time (APTT), Prothrombin Time (PT), and some institutes include fibrinogen assays too in this test. The detection system can be categorized into two types such as photo-optical method and electromechanical method.
23 Pages(5750 words)Dissertation
Diabetes
It is established that “autoimmunity is the predominant effecter mechanism” for the development of the illness (Van Belle, Coppieters, & Von Herrath, 2011, p. 79). Van Belle, Coppieters, and Von Herrath (2011) suggest that while autoimmunity may create the potential for developing the illness, it is environmental factors that hold the true causality.
12 Pages(3000 words)Case Study
Medical
I would like to assure you that you don’t need to be anxious regarding this issue. Hormonal problems are fairly common especially in women of reproductive age group. You need to understand that there are several different hormones in our body which are essential for different body functions.
3 Pages(750 words)Essay
Monitoring the glucose level as well as alteration in expression of m-RNA and protien levels of glucose transporters(GLUT4) in d
Vitamin D is found to regulate the insulin secretion along with the calcium ions. Vitamin D deficiency results in the imbalance of the parathyroid hormone in the body and results in the increase in the calcium level and the increase of calcium inhibits the insulin activity.
4 Pages(1000 words)Research Proposal
Arteriosclerosis
Unhealthy arteries kill more and more people not just in America, but in several parts of the world. Arteriosclerosis is a serious, dangerous condition which can possibly lead to heart attack and other forms of cardiovascular diseases. Worldwide, cardiovascular (CV) disease is known as the common cause of mortality as well as one of the top causes of disability.
10 Pages(2500 words)Essay
Protien
The most possible sequence given was based on codon usage database generated using all the E. coli coding sequences in GenBank. The presence of repeats, palindromic, sequences were
4 Pages(1000 words)Essay
Master project ( protien intake and bone lose) in elderly
Older adults are very often found to suffer from hip fracture. Protein intake is immensely potent to reduce undesirable outcomes from the fracture There exists a huge debate over the effectiveness of protein intake on bone health in older adults. While in one hand a number
10 Pages(2500 words)PowerPoint Presentation
Let us find you another Essay on topic Protien refording for FREE!
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us