Polymerase chain reaction (PCR), a molecular technique that amplifies a specific gene fragment offers a more rapid, sensitive and specific method of detecting and identifying pathogens. The purpose of this experiment is to detect the presence of viral genome in the genomic DNA…
However, no viral DNA was detected in MRC5 cell line. In conclusion, PCR is an effective tool for the detection of viral genome in infected host cells.
Conventional laboratory methods of identifying disease-causing pathogens often involve morphological characterization and antigen detection (Lee et al., 2009; Nitzan et al., 2009; Cicek et al., 2007). However, the traditional morphological examination which relies heavily on culture techniques, presents a serious laggard, on top of contamination problems (Candrian 1995).
The emergence of molecular diagnostic tools have circumvented and altered the limitations brought about by the conventional diagnostic techniques (Lion et al., 2006). Specifically, genome-based techniques are increasingly becoming popular due to their high specificity and sensitivity. One tool that has revolutionized the field of clinical diagnostics is polymerase chain reaction (PCR), an enzymatic procedure which amplifies a specific fragment of DNA or RNA (Lion et al., 2006). Since PCR makes use of nucleic acids to detect the presence of organisms, it is extremely useful in the identification of notoriously difficult-to-detect pathogens like viruses.
It has been established that human papilloma virus (HPV) infection is a necessary precursor for the onset of cervical cancer (Stanley 2010). In fact, approximately 90% of cervical cancer cases can be accounted for by HPV types 16 and 18. Since HPV infection is very common with a lifetime risk of infection of 50-80%, its accurate diagnosis is very crucial (Stanley 2010).
. The purpose of this experiment is to detect the presence of viral genome using polymerase chain reaction. Specifically, the experiment seeks to compare the genomic DNA extracted from HeLa cells, a cervical cancer cell line and MRC5 cells derived from a 14-week old fetal lung tissue in order to confirm that HeLa cells contain human papilloma virus (HPV) 18 DNA. The HeLa ...
To keep the sample from getting oxidized, nitrogen was introduced into the furnace while it was made sure, that no oxygen entered it. Tin’s melting point was virtually determined through its transition from solid to molten state. Tin was then shifted into a coffee can.
The genetically modified reference standards were used as controls and the samples were analyzed using agarose gel electrophoresis. GMOs was not identified in all of our soy beans samples with PCR method, we rejected our null hypothesis that stated GMOs was present in our soy beans samples.
In total 98 subjects took part in the study.
The intra-dermal injections are useful for allergy and tuberculosis tests. The commonly followed procedure is the bevel up technique wherein the bevel of the syringe faces against the skin surface before inserting the needle.
ast tumour and normal tissue specimens, and compare the amplified target cDNA to a constitutively expressed house-keeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Dysregulated cell cycle control is a primary mechanism of cancer (Deshpande, Sicinski & Hinds,
Salmonella typhimurium is a leading cause of gastroenteritis in humans
This experiment tested the histidine requirements of the auxotrophic mutant strains and their spontaneous reversion rate. The experiments were carried using either wild
After DNA is extracted from the maize, gel electrophoresis is done using agarose gel for the visibility of the DNA strands. A photograph of the amplified genes is then taken using a UV Trans illuminator.
The photograph will provide a hard or soft copy of the DNA strands that
What this implies is that there are several different activities and tasks that are put together to form the collective concept of a start-up. One of these key activities is a market research, which serves as a blueprint for understanding the dynamics of the
between meals of breakfast and lunch meals or lunch and dinner.Furthermore, I noticed that I eat a lot of snacks during my mobile part of the day i.e. when moving from one point to another during my day to day
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