The PCR –RFLP method was used for the analysis of the different fruits in the fruit juice. The PCR heteroduplex system was successfully applied to the LOC and was found to show greater sensitivity for the identification of the fruit juices. After this process, random reannealing of the amplified DNA was done based on Hardy- Weinberg law. The heteroduplexes obtained from these studies have proved that the heteroduplexes were underestimated at the lab-on-a-chip method. The differential data interpretation is done to obtain better results. (Scott and Knight 2009).
DNA profiling method was developed for the fruit samples. Different DNA extraction methods were practiced for the isolation of the genomic DNA from the fresh fruits. Some of the DNA extraction methods are CTAB- based DNA extraction method and some kits such as Nucleon Phytopure plant DNA Extraction kit, DNeasy Plant mini kit and G2 buffer & Genomic –tip 20/G kits can be used for the extraction of the DNA from the given fruit juices. SSR markers are used for the identification of the DNA from the given samples. The SSR analysis was able to reveal that the genotypes were the same for the dried fruits and the fresh juices. The DNA samples obtained from the canned and processes foods will be very small of 500 bp. (Yamamoto et al. 2006).
These DNA samples can be easily amplified using the high quality primers. In the pear fruit DNA sequence there are 15 SSR sequences and among them 9 SSR markers were obtained from the fruit juice containing pear. The reason for this could be that the DNA obtained from the fruit juices were around 150 base pairs and they were very small for amplification. The DNA profiling and the cultivar methods were found to be very useful for the identification of the DNA of the fruits from the fruit juices. (Yamamoto et al. 2006).
Orange juice is one of the most