Abstract of Microbiology Paper Osorio L, Ramirez M, Bonelo A, Villar LA, Parra B. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol J. [Internet] 2010 [cited 2011 March 24]; 7: 361. Available from http://www.ncbi.nlm.nih.gov/pubmed/21134275 Dengue is one of the vector borne diseases that spreads rapidly in the urban regions of tropical and subtropical countries…
Basically, 3 are 3 laboratory tests useful for the diagnosis of dengue infection and they are viral isolation in culture, detection of viral RNA, and specific IgM/IgG antibodies in paired sera. The gold standard for diagnosis is a combination of these 3 tests. However, it is not practice to do all the 3 tests in a patient at a particular setting. Viral isolation is expensive, needs technology and results are obtained after 6 - 10 days, which ia very late for initiation of management and hence this test cannot be used regularly. PCR technique results are ofcourse fast, but are expensive and not available in all clinical settings. Immunochromatographic and ELISA tests for the detection of IgM/IgG antibodies which give results within minutes or few hours, but these antibodies can be detected only after 4-5 days of onset of the disease. Also, paired sera samples showing seroconversion or a fourfold titer increase are essential to confirm diagnosis. ...
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