INTRODUCTION Presumptive blood tests are important, especially in the field of Forensics. The presence or absence of blood may corroborate the statement of a witness. Its specificity lies on the reaction of the chemicals to hemoglobin, a major component of blood…
The specificity of this test lies on the fact that hemoglobin is only contained in blood. This change in pH is then detected by phenolphthalein, and the solution turns into pink quickly, or leuchomalachite green, which turns the solution into green (Houck and Siegel, 2010; Tobe et al., 2007). However, there are a few considerations in using these substrates. First, because phenolphthalein is a pH indicator, phenolphthalein reacts with any basic solution. It is thus important that the addition of LMG or PPT prior to the addition of hydrogen peroxide do not yield a color change. This ensures that any change in the solution results from the reaction of hemoglobin with hydrogen peroxide. Second, when the phenolphthalein is exposed too long in the solution, a spontaneous oxidation occurs causing the colorimetric reaction to occur (Houck and Siegel, 2010). The color change indicating presence of blood must thus be quick. Third, substances in fruits and vegetables may act like a peroxidase (Tobe et al., 2007). MATERIALS AND METHODS Sample FS-26-07 was tested for the presence of blood using leuchomalachite green (LMG) and phenolphthalein (PPT). Two set-ups were prepared. Each set-up consists of three spot plates each containing a strip of 1) positive control, 2) negative control, or 3) FS-26-07. The spot plates in the first set-up were added with two drops of LMG, while those of the second set-up were added with the same amount of PPT. ...
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Since 19th century scientists were aware of some unique identity of an individual which distinguish from others, like finger prints. In 1970, DNA was established as key element in human life and which makes individual as unique creature. Variability in DNA sequences among individual to individual have attracted forensic experts to develop technique based on DNA to identify individual or criminal similar to finger prints.
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Another related objective was to apply equations for basic projectile motion in determining how high a rocket can fly. Since launching the model rocket, this is considered a projectile motion influenced by height of launch pad,
For the first cycle, the mass of the water alone is the mass of the crucible lid and hydrate, less the mass of the crucible lid and residue after heating. That gives 22.678 – 22.460 = 0.218. The mass of the hydrated salt is the mass of the crucible lid and hydrated salt, less
o calibrate the pressure transducer and then perform an uncertainty analysis and compare your pressure transducer calibration to the manufacturer’s calibration.
1. Excite the pressure transducer, with a voltage of 10v. The figure above had J17 and J18 as AO (Analog Out). It
For this experiment a cuvette, calorimeter, and a color of your choice for the dye solution, A.To begin the experiment, clean the cuvette. Wash out the cuvette three times with water, and then three times with solution. Once
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