Prosthetic Dentistry

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A study of colonization of MRSA strain on denture surfaces by Tawara et al, 1996, found 10% of the denture wearing patients carried MRSA on their dentures, which was difficult to eradicate using conventional denture cleaning agents. In order to find out which denture surface material, if any, between acrylic resin, molliplast and viscogel, is less supportive of the growth of MRSA16, equal number of these cells were grown on different denture surface materials and the colony forming units per ml of the cellular suspension from these surfaces were assessed on biofilms at 3, 24 and 72 hours.


Several chemical denture cleansers are commercially available. Since MRSA isolate of Staphylococcus aureus is resistant to penicillin's, including methicillin, we investigated the bactericidal effects of two different denture-cleansing agents including Milton (composed of 2% sodium hypochlorite, Ceuta Healthcare, Bournemouth, UK) and Perform (composed of Potassium Peroxomonosulphate, Sodium Benzoate, Tartaric Acid, Schlke and Mayr UK Ltd., Sheffield, UK) on the growth of MRSA 16 on the denture surface acrylic and visco-gel.
Results from a 10 minute exposure to cleanser, Milton and perform, on the survival of MRSA 16 biofilms at 3, 24 and 72 hours of growth suggest a significant decrease in the survival of MRSA 16 on acrylic surface at all time points viz., 3, 24 and 72 hours of growth, as assessed by colony forming units per ml of cell suspension from this surface (Figure 2). ...
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