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Prosthetic Dentistry - Essay Example

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Prosthetic Dentistry

Several chemical denture cleansers are commercially available. Since MRSA isolate of Staphylococcus aureus is resistant to penicillin's, including methicillin, we investigated the bactericidal effects of two different denture-cleansing agents including Milton (composed of 2% sodium hypochlorite, Ceuta Healthcare, Bournemouth, UK) and Perform (composed of Potassium Peroxomonosulphate, Sodium Benzoate, Tartaric Acid, Schlke and Mayr UK Ltd., Sheffield, UK) on the growth of MRSA 16 on the denture surface acrylic and visco-gel.
Results from a 10 minute exposure to cleanser, Milton and perform, on the survival of MRSA 16 biofilms at 3, 24 and 72 hours of growth suggest a significant decrease in the survival of MRSA 16 on acrylic surface at all time points viz., 3, 24 and 72 hours of growth, as assessed by colony forming units per ml of cell suspension from this surface (Figure 2). ...
While there seems to be some recovery of growth at 24 and 72 hours after the initial decrease in survival at 3 hours using Milton for 10 minutes, Perform was very efficient in almost complete eradication of MRSA at 72 hours post treatment.
Table 1
MRSA % kill
3 hours
24 hours
72 hours
Milton 10 min
99.97
99.88
99.95
Perform 10 min
99.97
99.90
100.00

Effect of denture cleansers Milton and Perform on growth of MRSA 16 on visco-gel:
Results from a 10 minute exposure to cleanser, Milton and perform, on the survival of MRSA 16 biofilms at 3, 24 and 72 hours of growth suggest a significant decrease in the survival of MRSA 16 on visco-gel surface at all time points viz., 3, 24 and 72 hours of growth, as assessed by colony forming units per ml of cell suspension from this surface (Figure 3). Perform was more effective in decreasing the survival of the cells at 3 and 24 hours. However, both the reagents were similarly effective at 72 hours of growth of MRSA 16 on visco-gel (Figure 3). Calculation of percent cell killing by these cleanser as seen in Table 2 clearly shows that these agents, particularly, Perform, are very effective in controlling the MRSA 16 strain.

Table 2
MRSA % kill
3 hours
24 hours
72 hours
Milton 10 min
42.86
68.11
99.58
Perform 10 min
99.81
99.34
99.53

Discussion:
The ultimate goal of dental prosthetics is the development of surface material that is bactericidal and least supportive of growth of any type of bacteria, particularly, that of super bug, MRSA 15 and 16. Our experiments to identify such an ideal surface lead to identification of molliplast as a denture surface that is least supportive of the growth of MRSA ...Show more

Summary

A study of colonization of MRSA strain on denture surfaces by Tawara et al, 1996, found 10% of the denture wearing patients carried MRSA on their dentures, which was difficult to eradicate using conventional denture cleaning agents. In order to find out which denture surface material, if any, between acrylic resin, molliplast and viscogel, is less supportive of the growth of MRSA16, equal number of these cells were grown on different denture surface materials and the colony forming units per ml of the cellular suspension from these surfaces were assessed on biofilms at 3, 24 and 72 hours…
Author : bergnaumwinston
Prosthetic Dentistry essay example
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