Prosthetic Dentistry - Essay Example

Several chemical denture cleansers are commercially available. Since MRSA isolate of Staphylococcus aureus is resistant to penicillin's, including methicillin, we investigated the bactericidal effects of two different denture-cleansing agents including Milton (composed of 2% sodium hypochlorite, Ceuta Healthcare, Bournemouth, UK) and Perform (composed of Potassium Peroxomonosulphate, Sodium Benzoate, Tartaric Acid, Schlke and Mayr UK Ltd., Sheffield, UK) on the growth of MRSA 16 on the denture surface acrylic and visco-gel.

Results from a 10 minute exposure to cleanser, Milton and perform, on the survival of MRSA 16 biofilms at 3, 24 and 72 hours of growth suggest a significant decrease in the survival of MRSA 16 on acrylic surface at all time points viz., 3, 24 and 72 hours of growth, as assessed by colony forming units per ml of cell suspension from this surface (Figure 2). . While there seems to be some recovery of growth at 24 and 72 hours after the initial decrease in survival at 3 hours using Milton for 10 minutes, Perform was very efficient in almost complete eradication of MRSA at 72 hours post treatment.

Table 1MRSA % kill3 hours24 hours72 hoursMilton 10 min99.9799.8899.95Perform 10 min99.9799.90100.00Effect of denture cleansers Milton and Perform on growth of MRSA 16 on visco-gel: Results from a 10 minute exposure to cleanser, Milton and perform, on the survival of MRSA 16 biofilms at 3, 24 and 72 hours of growth suggest a significant decrease in the survival of MRSA 16 on visco-gel surface at all time points viz., 3, 24 and 72 hours of growth, as assessed by colony forming units per ml of cell suspension from this surface (Figure 3).

Perform was more effective in decreasing the survival of the cells at 3 and 24 hours. However, both the reagents were similarly effective at 72 hours of growth of MRSA 16 on visco-gel (Figure 3). Calculation of percent cell killing by these cleanser as seen in Table 2 clearly shows that these agents, particularly, Perform, are very effective in controlling the MRSA 16 strain. Table 2MRSA % kill3 hours24 hours72 hoursMilton 10 min42.8668.1199.58Perform 10 min99.8199.3499.53Discussion: The ultimate goal of dental prosthetics is the development of surface material that is bactericidal and least supportive of growth of any type of bacteria, particularly, that of super bug, MRSA 15 and 16.

Our experiments to identify such an ideal surface lead to identification of molliplast as a denture surface that is least supportive of the growth of MRSA