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Seed Germination - Lab Report Example

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Seed Germination

It first reduces imbibition of water because of the lowered osmotic potential of the medium (Bliss et al., 1985). Second, it causes toxicity; that is, it changes enzymatic activity, hampers protein metabolism, upsets plant growth regulators balance, and reduces the utilization of seed reserves. It may elicit changes at ultrastructural, cellular and tissue, or even at organ levels (Dell'Aquila & Spada, 1993). Salinity interacts with certain plant and environmental factors during germination. Among these plant factors are seed coat, dormancy, seed age, seed polymorphism, and seedling vigor. Environmental factors include temperature, light, availability of water, and oxygen (Pessarakli, 1999).
In this experiment, it will be determined if salt will interfere with the rate of germination of soybean seeds. To assure that all other factors are held constant, a standard germination test will be undertaken under optimum conditions for seed germination.
2. Five rectangular plates (12"x5") will be lined with paper towels pre-moistened by different solutions. Plate A is the control and it will be moistened with distilled water. Plate B will be moistened by the 0.5M NaCl solution, Plate C will be moistened with 1M NaCl solution, Plate D will be moistened with 2M NaCl solution and Plate E is moistened with 3M NaCl solution.
4. 3. Thirty soybean seeds will be distributed evenly in the rectangular plates and the plates will be placed in the greenhouse.
4. In the next three days, the paper towels in the plates will be moistened twice a day (10 AM and 4 PM) with the appropriate salt solution to prevent water loss due to evaporation. Also, the germinated seeds in each plate will be counted and recorded. . For this experiment, we will define germination as the breaking of the seed coat and the emergence of the radicle.
5. In the fourth day, the number of germinated will be totaled and compared.
Results and Discussion
Table 1. Number of Germinated Soybean Seeds in Different Plates per Day
Plates
Day 1
Day 2
Day 3
Day 4
Total
Plate A (Control)
5

6


4


2


17
Plate B (0.5M NaCl Solution)
6
5

4

3

18
Plate C (1M NaCl Solution)
0
3
2
1
5
Plate D (2M NaCl Solution)
0
1
2
1
4
Plate D (3M NaCl Solution)
0
0
0
1
1

With the obtained results, the Chi-Square Test will be used because it will be compared how well an observed ...Show more

Summary

Seed germination is a complex phenomenon involving many physiological and biochemical changes and leading to the activation of embryo (Bewley & Black, 1985). Seeds vary in their rate of germination. In normal conditions, soybean seeds should germinate in one to two days.
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Seed Germination essay example
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