Conjunctiva in Contact Lens Wear

College
Research Proposal
Science
Pages 5 (1255 words)
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The objective of the research program is to make a detailed examination of the bulbar and superior palpebral conjunctiva in contact lens wearers and age- and sex-matched non-lens-wearing control subjects, using a Heidelberg Laser Scanning Confocal Microscope by a random study of 40 equally divided contact and non contact lens wearers (control group) to study the alteration whether increase or decrease in the superficial conjunctival epithelial cell density.

Introduction

In a study conducted by Aragona P, Ferreri G, Micali A, Puzzolo D. in 1998 to detect the morphological changes of the conjunctival epithelium in contact lens wearers evaluated by impression cytology, it was found that cellularity, nucleus to cytoplasm ratio and goblet cell distribution were altered. In another study by Knop E, Brewitt H. in 1992 , where they discovered using the impression cytology technique on contact lens wearers that enlargement of the conjunctival cells took place as well as flattening of their cells. They also noted a decrease in the number of goblet cells. The late finding was contrary to what Connor C.G., Campbell J.B., Steel S.A., Burke J.H. have found in 1994 of an increase of goblet cells in daily contact lens wearers. As the conjunctiva has an important functional demand on the eye helping in keeping the eye moist and its cleansing effect through tears, it is thus an important first defence against debris and pathogens. Direct in vivo observation of the conjunctiva at a cellular level using the confocal microscope is likely to provide important new insights into the impact of contact lens wear on the ocular surface.
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