Solid-phase synthesis

College
Lab Report
Science
Pages 3 (753 words)
Download 0
Gly has a vast pK range and can be covalently linked to the resin at any pH value. Glu has quite acidic isoelectric point (pI) (= 3.1), and for all reactions in aqueous solutions (Boc and DCCD derivatization) it is required to keep pH close to 3.0. Once Glu is linked, Lys has very alkaline pI (= 9.8) and thus the resin should be washed with a buffer of pH 10.0…

Introduction

All blocking reactions (Boc, DCCD and benzyloxycarbonic acid) need to be carried out in aqueous phase at this pH. Subsequently, for Gly and Ala, pH can be brought back to neutral or slightly acidic (pH 5.0-7.0). Orthogonal protection is a method to protect other than N' and C' terminals, the COOH, NH2, and other groups which may affect peptide bond formation or cause stearic hindrance such as -OH, -SH etc. Depending on the groups to be protected (here Lys) chemicals can be chosen. As coupling of amino acids through a peptide bond is enderogenic reaction, they need to be activated by DCCD before coupling.
...
Download paper
Not exactly what you need?

Related papers

Role of Phytooestrogen in the Prevention of Breast Cancer
They have been suggested to have anticarcinogenic potential (Boccardo et al, 2006). Plant lignans are currently being widely studied for their potential benefits for human health as their consumption has been correlated with lower risks for developing chronic diseases, such as breast cancer and coronary heart disease. However, studies of some classes of lignans, in particular the furofurans, are…
Abalytical Toxicology
This still remains a great challenge for analytical chemists and may rely on identification of different target compounds. In the case of precursor drug administration, the parent compounds are usually detectable only for a short time, before being fully metabolised or decomposed. For this reason parent compounds can rarely be used for differentiating. Specific metabolites are usually detectable…
Solid Work Simulation
The concept of this analysis lies in the fact that the elements have the nods which would behave in the same way under different load conditions, thus it would be possible analyzing the three degrees of freedom of every nod can be fully translated by notation. Here probably, need to know how different load types will relate to the bending moments. The relationship between moment and loads.…
Amino Acide
Polymerization is a common reaction, due to excessive use of amino acids to ensure coupling during each synthesis phase. As a result, a series of deprotection phases are added to the synthesis process which takes the following form:…
Solid-phase synthesis
All blocking reactions (Boc, DCCD and benzyloxycarbonic acid) need to be carried out in aqueous phase at this pH. Subsequently, for Gly and Ala, pH can be brought back to neutral or slightly acidic (pH 5.0-7.0). Orthogonal protection is a method to protect other than N' and C' terminals, the COOH, NH2, and other groups which may affect peptide bond formation or cause stearic hindrance such as -OH,…
Asymmetric Synthesis
Hence, it is undeniably interesting to study the catalytic asymmetric synthesis of cinchona alkaloids , which have also been helpful in man's daily living. This includes its role in the production of food flavorings and medicines for different ailments like malaria.…
Environmental Science Bachelor Essay
This essay takes the view that it is already too late to address the current colossal effects of pollution on environment and the only way to redeem ourselves is to adopt technology as an aide to developing cleaner solutions. Scientists, policymakers, and laypeople are beginning to evaluate and understand the gravity of the situation and interpret data about environmental change in a new light:…