Polymerase Chain Reation

Masters
Lab Report
Science
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This is an example of 'Point Mutation: Transversion' wherein 'C' in the control patient gene is replaced by 'G' in the affected patient gene, leading to a slightly altered protein, consequences of which include:
Samples 1, 3, 4, 5, 5, 6, 9 & 11 can be considered as heterozygous for the simple reason their SSCP profiles seem to be a combination of profiles of the other samples…

Introduction

Additionally, each of the bands observed in the SSCP profiles of Samples 1, 3, 4, 5, 5, 6, 9 & 11 can be correlated with those observed in the SSCP profiles of samples 2, 10 & 12 (Allele profile 1; the control type) and/or samples 7, 8 & 13 (Allele profile 3; one of the mutant SSCP alleles). Such a situation is generally seen in heterozygosity.
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