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Microbiology of Diabetic Rats
Pages 38 (9538 words)
In the present study, we have sought to investigate the nature and count of the gut microbiota in diabetic condition. The GI tract shelters a colony of microbes, which help in maintaining the overall physiological health in mammals. Experiments were conducted on rats to bring about the desired results.
We underwent through intricate aseptic techniques for preparing microbial cultures from the commensal population of microbes in the gut isolated from the diabetic and normal rats for recording their count from the extent of lactate, acetate, and glucose production based on four kinds of bacteria, Staphylococcus aureus, Enterobacteriaceae sp, Lactobacillus sp, and Enterococcus sp. We concentrated our entire assay on aseptic cultures of these microbes grown on various kinds of agar each suitable for the cultures of a particular bacterial strain. The basic kinds of agar displays that were used included, Mannitol Salt Agar for Staphylococcus aereus, MacConkey Agar for Enterobacteriaceae sp, Slanetz & Bartley agar for Enterococcus sp and MRS Agar for Lactobacillus sp. Subsequently we observed the commensal microbial growth in 4 and 8 week diabetic rats along with that in normal rats for obtaining comparative figures through a number of confirmation tests including Catalase test, Coagulase Test, RAPIDEC Staph Test, Oxidase test and API 20E Test. Significant findings showed that the diabetic rats weighed more than the normal rats. The stool of the Ileum and colon of normal and diabetic rats were sent to investigate the microbial aberrancies, after the induction of streptozotocin. ...
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