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Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator - Lab Report Example

Masters
Lab Report
Science
Pages 10 (2510 words)
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Summary

Performing a simple experiment using the Bromcresol-Green(BCG) method for Albumin concentration determination in human serum an observation was made that upon the addition of protein globulins an interference occurs raising the result significantly. This finding concurs with a literature review that was performed…

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Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator

Albumin's molecular structure was reported from research in the past 30 years. Albumin's clinical importance was discussed, explaining that the test for Albumin is not a marker for any specific disease, while it is an important indicator for the physiological condition of the body. It was explained that an abnormally high result for Albumin needs to resolved and at face-value is considered a spurious result. The cause of elevated albumin levels are usually a result of accompanying serum proteins, acute phase infections, elevated immunoglobulins(found in multiple myeloma), or autoimmune issues. The BCG method was thoroughly researched. It is a valuable test for screening, when performing mass analysis of blood work in large clinical laboratories. It has fallen out of favor with laboratory clinicians for fear that the test can mask hypoalbunemia and low readings. BCG is an acid-base indicator, its acid to base color change is from yellow to blue, with a range in pH from 4.2-5.6. The detection of hypoalbunemia is always important to a physician. Low Albumin readings are indicative of a deteriorating condition in the body. An alternative to the BCG indicator is Bromcresol Purple(BCP), also an acid–base indicator. A comparison studies have been performed of the two methods. ...
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