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Interferences in the Assay of Serum Albumin
Pages 15 (3765 words)
Performing a simple experiment using the Bromcresol-Green(BCG) method for Albumin concentration determination in human serum an observation was made that upon the addition of protein globulins an interference occurs raising the result significantly. This finding concurs with a literature review that was performed.
It was explained that an abnormally high result for Albumin needs to resolved and at face-value is considered a spurious result. The cause of elevated albumin levels are usually a result of accompanying serum proteins, acute phase infections, elevated immunoglobulins(found in multiple myeloma), or autoimmune issues. The BCG method was thoroughly researched. It is a valuable test for screening, when performing mass analysis of blood work in large clinical laboratories. It has fallen out of favor with laboratory clinicians for fear that the test can mask hypoalbunemia and low readings. BCG is an acid-base indicator, its acid to base color change is from yellow to blue, with a range in pH from 4.2-5.6. The detection of hypoalbunemia is always important to a physician. Low Albumin readings are indicative of a deteriorating condition in the body. An alternative to the BCG indicator is Bromcresol Purple(BCP), also an acid-base indicator. A comparison studies have been performed of the two methods. When compared to a benchmark analytic method Capillary Zone Electrophoresis(CZE), the positive bias for BCG was 3.77, while BCP faired much better with 0.67. Our experiment produced linear curves needed in detection and quantification of albumin concentrations. We demonstrated the effect on accompanying globulins elevating the result in BCG determinations of albumin. ...
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