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Pages 34 (8534 words)
This paper introduces a research project that shall investigate the expression patterns of the NKCC (Sodium-Potassium-coupled Chloride) Cotransporters in a newly-developed murine neuronal cell line CAD (caspase-activated deoxyribonuclease) to try and establish molecular identity and other traits, such as genetic implications, of the two cotransporters in this subfamily - NKCC1 and NKCC2…
Of these NKCC1b is also known to be found in brain RNA (Gamba, 2005). It is noted here, though, that the two isoforms of the NKCC1 cotransporter is found only in the European eel (Anguilla anguilla) as per research of Cutler and Cramb, 2002. Nevertheless, there is ample evidence that NKCC1, in human and other mammalian species, is functionally implicated in CNS cells. It is observed by Gamba, 2005, that the NKCC1 cotransporter is activated by receptors and assists in neurotransmission by driving anions into the cell. It is also observed by Strange et al, 2000, that the work of the NKCC1 cotransporter complements that of the KCC2 one. The choice of the culture medium, the neuronal-specific CAD cell line, and the somewhat CNS-specific NKCC1 dovetails perfectly for a research attempt that seeks to establish new facts on the molecular identity and other expression patterns of these unique electroneutral cotransporters in cells of the central nervous system (CNS). G. Gamba's excellent 2005 review article on these cotransporters has been extensively used in this paper because it is the most comprehensive document prepared to date being inclusive of all aspects described so far.
RT-PCR analysis was carried out ...
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