Genetics - Essay Example

Only on StudentShare

Extract of sample
Genetics

This was done to obtain the RAD51+/- cells (EMBO, 1998). Then, the RAD51+/- cells have been transpected with conditional human Rad51 expression constructs to obtain RAD51+/- cells carrying the constructs at random sites on the chromosome (RAD51+/-/HsRAD51)." (, 1998) Finally, the RAD51 construct that contained the blasticidin was then transpected into "several RAD51+/-/HsRAD51 clones to isolate RAD51-/-/HsRAD51 clones." (EMBO, 1998)
The targeting process is shown in figures one and two. In order to target the necessary genes, a probe and southern blot analysis were used to indicate the knockout constructs. Samples of the cells and genetic material were loaded onto five different lanes and were combined with SDS-polyacrylamide gel. The three samples were the wild-type DT40, which was loaded onto lane 1, the RAD51+/-, which was loaded onto the second lane, a RAD51+/- clone that contained the human Rad51 transgene, which was loaded onto the third lane, #110 RAD51-/- clone was placed onto the fourth lane, and a human B lymphocyte line Ramos was loaded onto the fifth lane (EMBO, 1998).
The targeting probe was constructed of A chicken RAD51 (GdRAD51) cDNA, and this probe was used to isolate the genomic clones that were of the RAD51 locus. These clones were, in part, sequenced to determine the position of the exons. ...Show more

Summary

In order to target the RAD51 gene, a very specific experimental procedure was followed, and it is clearly outlined in this paper. This procedure was designed to produce RAD51 mutant clones, and this was done by causing a disruption to both of the alleles in the RAD51 gene…
Author : annabell99
Genetics essay example
Read Text Preview
Save Your Time for More Important Things
Let us write or edit the essay on your topic
"Genetics"
with a personal 20% discount.
Grab the best paper

Related Essays

GENETICS
Generally, if there is a heritable factor, then the cancer will occur earlier in life than a sporadic condition. However, it is important to note that people do not inherit breast cancer, but rather an increased likelihood of having the disease . Around 20 – 30% of women who have breast cancer have the disease occurring within their family, however there are also many environmental risk factors, such as use of alcohol or exposure to radiation .
14 pages (3500 words) Essay
Behaviour Genetics
This is a discussion on the issues dealing with behaviour genetics and ethics that has been a major part of the news in recent months. Several areas of the arguments would deal with the genetics and behavioural changes, whether behaviour is species specific, whether behavioural changes relate to changes in biological structures and processes and the evolutionary history or DNA sequencing that are related to behavioural genetics.
10 pages (2500 words) Essay
Genetics
ant trait) and the other that is a short plant (recessive trait) is expected to produce an F1 (first) generation of plants that are al tall because the allele of tallness would dominant to shortness in the offspring produced. However, all plants produced in F1 generation would
4 pages (1000 words) Essay
Genetics
The human cells consist of 46 chromosomes. Of these, two are sex chromosomes and 22 pairs are autosomal chromosomes. The sex of a human being is
8 pages (2000 words) Essay
GENETICS
Here a gene could be cut into small fragments, and a fragment from another gene having a different make up, can be inserted between the cut fragments, and then reattached together to form a completely new gene. There are many
12 pages (3000 words) Essay
Human Genetics
According to the Australian Law Reform Commission, "Human genetic research generates knowledge with the potential to improve individual and
1 pages (250 words) Essay
Genetics 1
The questions are undertaken based on credible experiments. The probability of the parents getting 10 children where 3 that are normal for both traits, 3 that have iron overload, but normal blood calcium and 4 that exhibit both iron overload and
2 pages (500 words) Essay
Genetics
Each gene codes for a specific protein in the body by specifying the order that the amino acids needs to be joined together. The function of the DNA in the body is carrying genetic code of information that determines the characteristics of a living thing but it is
10 pages (2500 words) Essay
Genetics
If you fertilize a trisomic Jimson weed plant, what zygotes would be formed? Tell which zygotes are diploid and which are trisomic. Assume that only the univalent mode occurs during meiosis; 2. Down syndrome can result from a Robertsonian
3 pages (750 words) Essay
Genetics
If you fertilize a trisomic Jimson weed plant, what zygotes would be formed? Tell which zygotes are diploid and which are trisomic. Assume that only the univalent mode occurs during meiosis; 2. Down syndrome can result from a Robertsonian
3 pages (750 words) Essay
Get a custom paper written
by a pro under your requirements!
Win a special DISCOUNT!
Put in your e-mail and click the button with your lucky finger
Your email
YOUR PRIZE:
Apply my DISCOUNT