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Epstein-Barr Virus - Essay Example

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Epstein-Barr Virus

Transmission could occur contacting the saliva of an infected person, such as persons with infective mononucleosis, not usually through air or blood. Although healthy people can carry and spread the virus, known as primary reservoir for person-to-person transmission, so preventing the cause entirely is unachievable. During routine diagnosis, primary infection of EBV can be established through serologic testing or specific laboratory, funding elevated blood cell increased lymphocytes positive "mono spot" test or specific antibody high levels, although as indirect detection of EBV, could be sighs in blood and lymphoid tissue where leading through research we consider them as a research tool. So symptoms compatible to infection mononucleosis and positive Paul-Bunnell heterophile antibody test result is diagnostic positive, also it is relevant to maintain on considerably high levels of heterophile antibodies, which are seen during first month of illness. Patient with infective monuclesosis show large expansion of EB specific CD8 T cell in the blood and so while latent infection of B cell poll is quickly controlled, virus shedding from lyticaly infected cell in the oropharynx remains high for several month. During primary infection response is localize to the tonsil. In acute IM, EBV specific effector inacute IM, EBV-specific effectors were poorly represented among CD8+ T cells in tonsil compared with blood, coincident with absence of the CCR7 lymphoid homing marker on these highly activated cells. In patients who had recently recovered from IM, latent epitope reactivities were quicker than lytic reactivities both to acquire CCR7 and to accumulate in the tonsil, with some of these cells now expressing the CD103 integrin, which...
The researcher states that although healthy people can carry and spread the Epstein-Barr virus (EBV), also called Human herpesvirus 4 virus, known as primary reservoir for person-to-person transmission, so preventing the cause entirely is unachievable. During routine diagnosis, primary infection of EBV can be established through serologic testing or specific laboratory, funding elevated blood cell increased lymphocytes positive “mono spot” test or specific antibody high levels, although as indirect detection of EBV, could be sighs in blood and lymphoid tissue where leading through research we consider them as a research tool. The researcher provides a detailed analysis of the Immunohistochemical method to identify and get rid of the Epstein barr virus. Tonsillectomy specimens are disaggregated to single-cell suspensions by testing apart the tissue and fine mincing. Mononuclear cells are isolated by purification over a Lymphoprep gradient per the manufacturer’s instructions. PBMCs are isolated in a similar manner from peripheral blood specimens taken at the time of tonsillectomy. In both cases, mononuclear cells are aliquoted and stored in liquid nitrogen. DNA was isolated from an aliquot of the tonsillar cells for HLA typing by sequence-specific oligonucleotide PCR analysis. Also it could be concluded in this research made, that through flow cytometry analysis of the analyses of the sample, ELISpot analyses of a sample, virus genom load analyses, and statical analyses that the method presented was successful. ...Show more

Summary

The researcher of this essay discusses and presents the Immunohistochemical method to identify Epstein barr virus, that is one of the most widespread human virus today which expands worldwide during human lives in tonsil tissue and got it removed from a patient…
Author : ashlynnmcdermot
Epstein-Barr Virus essay example
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