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Epstein-Barr Virus - Essay Example
Author : ashlynnmcdermot
Health Sciences & Medicine
Pages 4 (1004 words)
The researcher of this essay discusses and presents the Immunohistochemical method to identify Epstein barr virus, that is one of the most widespread human virus today which expands worldwide during human lives in tonsil tissue and got it removed from a patient…
The researcher states that although healthy people can carry and spread the Epstein-Barr virus (EBV), also called Human herpesvirus 4 virus, known as primary reservoir for person-to-person transmission, so preventing the cause entirely is unachievable. During routine diagnosis, primary infection of EBV can be established through serologic testing or specific laboratory, funding elevated blood cell increased lymphocytes positive “mono spot” test or specific antibody high levels, although as indirect detection of EBV, could be sighs in blood and lymphoid tissue where leading through research we consider them as a research tool. The researcher provides a detailed analysis of the Immunohistochemical method to identify and get rid of the Epstein barr virus. Tonsillectomy specimens are disaggregated to single-cell suspensions by testing apart the tissue and fine mincing. Mononuclear cells are isolated by purification over a Lymphoprep gradient per the manufacturer’s instructions. PBMCs are isolated in a similar manner from peripheral blood specimens taken at the time of tonsillectomy. In both cases, mononuclear cells are aliquoted and stored in liquid nitrogen. DNA was isolated from an aliquot of the tonsillar cells for HLA typing by sequence-specific oligonucleotide PCR analysis. Also it could be concluded in this research made, that through flow cytometry analysis of the analyses of the sample, ELISpot analyses of a sample, virus genom load analyses, and statical analyses that the method presented was successful. ...
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