Gene Splicing lab

College
Lab Report
Miscellaneous
Pages 5 (1255 words)
Download 0
Plasmid is a circular extra chromosomal DNA molecule found in bacteria which has the capability to replicate independent of the bacterial DNA.In gene splicing plasmids can be used as vectors for transmission of DNA into another cell. The genetic material to be introduced into the cell is integrated into the plasmid DNA and inserted into the cell.

Introduction


Any living organism that has had its genetic material altered by insertion of a gene from another organisms DNA using genetic engineering techniques, e.g., Drosphila melanogaster( the fruit fly) and Glo-fish, which is not a naturally occurring species
The same restriction enzyme has to be used to cleave both the parent DNA and the plasmid so that the free bases at the end of the fragment are complementary to the free bases in the plasmid at the site of the split. The attraction between the unpaired complementary bases will facilitate integration of the DNA fragment into the plasmid.
...
Download paper
Not exactly what you need?

Related papers

Gene One
The success that Gene One has had in eight short years has presented a quandary concerning the strategic path Gene One's future. Should Gene One execute of strategy of constancy and continuity Will this type of strategy keep Gene One's competitors from gaining ground on Gene One's share of the market place over the next several years After careful consideration of these perplexing questions, the…
Selfish Gene
Dawkins has established the foundation theory which was presented inthe volumeAdaptation and Natural Selection authored by George C. Williams's. He presented the termselfish geneas a means of articulating thegene-centric theory of evolution, which give out the idea that evolution, was best observed as acting ongenesand that selection at the organism level almost never dominates selection based on…
Gene Determination
Thus “for normal genital development, the gender 'message' must be communicated from the sex chromosomes to the gonads. The gonads must then manufacture appropriate hormones and the genital tissues and structures have to respond to these hormones. Any deviations along the way can cause ambiguous genitalia”. Some of the more specific causes of atypical genitalia that have been highlighted…
Kinetics Lab
4. Take a timer from the upper right hand corner of the screen and place it on the workbench. Wait till you obtain a round number on the timer to begin timing the reaction. Be ready to begin timing the reaction when you add the ammonium persulfate to the solution in the 250 mL beaker.…
Gene Expression
Before we start to Compare and contrast transgenic methods for manipulating gene expression between Drosophila and vertebrate animals let us discuss in brief about what …
Gene Manipulation
James Watson discovered the double helix structure of DNA. Due to which currently we have knowledge of our genes and thus makes it easier to manipulate them.…
Gene Splicing lab
Any living organism that has had its genetic material altered by insertion of a gene from another organisms DNA using genetic engineering techniques, e.g., Drosphila melanogaster( the fruit fly) and Glo-fish, which is not a naturally occurring species…