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Microbiology - Lab Report Example
Author : ylittel
Pages 30 (7530 words)
In the present study, we have sought to investigate variation of the GI tract microbiota in diabetic condition. The GI tract shelters a community of microbes, which help in maintaining the overall physiological health in mammals. Seven normal rats and five diabetic rats were used in the experiments…
We went through intricate aseptic techniques for preparing microbial cultures from the commensal population of microbes in the GI tract isolated from the diabetic and normal rats for recording their count from the extent of lactate, acetate, and glucose production based on four kinds of bacteria, Staphylococcus aureus, Enterobacteriaceae sp, Lactobacillus sp, and Enterococcus sp. The basic kinds of agar displays that were used included, Mannitol Salt Agar for Staphylococcus aureus, MacConkey Agar for Enterobacteriaceae sp, Slanetz & Bartley agar for Enterococcus sp and MRS Agar for Lactobacillus sp. Subsequently we observed the commensal microbial growth in 4 and 8 week diabetic rats along with that in normal rats for obtaining comparative figures through a number of confirmation tests including Catalase test, Coagulase Test, RAPIDEC Staph Test, Oxidase test and API 20E Test. Significant findings showed that the diabetic rats weighed more than the normal rats. The stool of the Ileum and colon of normal and diabetic rats were sent to investigate the microbial aberrancies, after the induction of streptozotocin. Consequently there was an incidental increase in GI tract microbes. The results showed that diabetes in rats were caused 3 days after streptozotocin.
Type 1 Diabetes (T1D)is a condition where the level of blood glucose rises due to absence of insulin. ...