Protein Concentration

Masters
Lab Report
Science
Pages 4 (1004 words)
Download 0
Proteins are large organic compounds built from 20 different amino acids. A protein will contain in the range of 50-2000 amino acid residues joined together by a chemical reaction that results in the formation of a peptide link and the release of a molecule of water…

Introduction

The two most common examples are -helix and -sheet (fig. 1)
Proteins are not completely rigid systems and they shift between several related structures while they perform their biological functions or roles. Such changes are often induced by the binding of a substrate to the protein's active site.
The Biuret reagent is composed of potassium hydroxide (KOH), copper (II) sulphate (CuSO4) and potassium sodium tartrate (KNaC4H4O64H2O). This reagent is used in a protein assay, a colorimetric test to determine protein concentration by detecting the Cu2+ ion with the help of spectrometric methods. In the presence of proteins, this blue reagent turns purple, while when there are short-chain polypeptides in solution it turns pink [MadSci Network].
A spectrometer is an optical instrument used to measure properties of light (intensity, polarisation, etc) over a specific range of the electromagnetic spectrum (in this experiment UV-Vis at a 560nm). The independent variable is generally the wavelength of the light expressed as nanometers. These instruments are used in spectroscopy to measure the interaction between radiation and matter.
In this experiment, UV/Vis spectroscopy is used to determine the concentration of a solution of proteins with the aforementioned Biuret agent. ...
Download paper
Not exactly what you need?

Related papers

Protein Misfolding Amyloid
But at pH 9.0 the intensity decreased sharply from beginning up to 70h and then attained a plateau. The decrease of 400u indicated huge conformational change leading to buried and /or quenched Trp residues.…
Protein
For analysis of complete genomes and to determine which gene families are present in the protein and which are not present, several techniques such as location of genes on the chromosomes, correlation with function or evolution, expansion of duplication of gene families, identification of missing enzymes, and presence or absence of biochemical pathways are considered. Large scale events that have…
C-reactive protein as a novel biomarker
This process leads to the formation of foamy macrophages and atheromatous plaques and, finally, to atherothrombotic disease. Atherosclerosis is associated with high morbidity and mortality.…
Protein Concentration
The two most common examples are -helix and -sheet (fig. 1)…
Protein Translocation
This also helps in identifying novel components for particular specific pathways and components common for all pathways. Mitochondrial proteins tend to be nuclear encoded and these proteins must be translocated to the compartments within the mitochondria (Copley, 1997; Berg 2002). The final localisation is determined with interaction of nascent polypeptides with inner and outer membrane…
Biomarkers
In aquatic environment, biomarkers depend upon the physical environmental conditions such as temperature, pH or salinity, as well as toxic concentrations of chemical pollutants or any combination of these.…
Physiology
This pathway consists of A & A fibers characterised with high velocity (30 to 110 m/sec) of impulse conduction. The dorsal column-lemniscal pathway has also high degree of spatial orientation of the nerve fibres; particularly the medial fibers convey the information from the lower parts of the body while the lateral fibers related to higher segments.…