Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria. It promotes the conversion of hydrogen peroxide, a powerful and potentially harmful oxidizing agent, to water and molecular oxygen…
The student feels that the more the concentration of the liver the more the enzyme that would result since more amount of oxygen would react with the hydrogen peroxide molecules. For accomplishing this, the student has taken five test tubes with the first test tube having twice the suspension as that of the fifth tube expecting that there would be twice the amount of oxygen given off. This seems that the student wants to prove that the amount of oxygen that would be evolved would be directly proportional to the amount of the liver suspension take. Since it cannot be decided in a single experiment the student repeats it three times to observe whether the desired changes have taken place in each experiment or not. The independent variable to be considered here would be the amount of liver suspension and distilled water and the dependent variable would be the amount of oxygen evolved. This experiment deals with quantitative analysis.
The overall experiment can be summed up by the following table
(Note: Exp indicates experiment)
It can be observed that there is a significant variation in the amount of oxygen evolved in the three experiments. The variation can be clearly understood observing the graph below
It can be inferred from the graph that there is a gradual decline in the amount of oxygen evolved considering the concentrations of the enzyme. (The x-axis represents experiment and the y-axis represents the amount of oxygen evolved). It is only in the second experiment that the discrepancy arises. It is highly probable that there might have been a replacement of the suspension. It can be clearly understood that there is a direct relationship between the amount of suspension and the amount of oxygen evolved.
The experiment is in fact a relatively simple one and can be performed easily if the conditions are ideal. The student has done it appropriately and has a good analytic skill. The prediction is purely based on understanding as it involves mainly quantitative analysis rather than the chemical reaction.
The student has opted the right materials for accomplishing this experiment. The only requirement would be to do the experiment under perfect conditions and conduct the experiment singly so as not to confuse with other experiments.
The following experiment would boost the above observation
Iftime is increased, then more hydrogen peroxide molecules will be split into water and ...
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(“Action Of Enzyme Catalase On Hydrogen Peroxide Lab Report”, n.d.)
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(Action Of Enzyme Catalase On Hydrogen Peroxide Lab Report)
“Action Of Enzyme Catalase On Hydrogen Peroxide Lab Report”, n.d. https://studentshare.net/science/294320-action-of-enzyme-catalase-on-hydrogen-peroxide.
The author provides an experiment, which has 1 independent variable, 1 dependent variable and 1 control variable. The independent variable is the various concentrations of hydrogen peroxide, while the dependent variable is the rate of reaction in terms of oxygen production. The control variable is the temperature of the room and standard pressure.
In general terms this was to introduce concepts related to enzyme kinetics. Introduction Enzymes are among several proteins generated in living cells to catalyze or accelerate the organism’s metabolic processes. Normally, enzymes are so selective and specific on the molecules they catalyze, referred to as substrate (Dixon, 1979).They normally do react specifically with a single substrate.
Using this model, we aim to understand the extent of change in the GI tract microbial count of streptozotocin injected diabetic rats compared to in normal rats based on a comparative assay of their glucose content, lactate content and the ratio of the GI tract to the body weights.
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Enzymes play a vital role
The composition of enzyme is primarily a complex protein molecule and function by catalyzing specific biochemical changes within the bodies of animals and plants such as digestion of food in animals and photosynthesis in plants. Enzymes acts on substrates and
By measuring how the temperature changes when H2O2 and a catalyst are mixed, it is possible to determine the enthalpy of decomposition H2O2. This experiment will determine the enthalpy of decomposition of H2O2 when it is mixed with potassium
They only change the rate of reaction (Kovarik & Allbritton, 2011).
The structures of enzymes comprise of a globular protein and a cofactor. The bonds holding the proteins in their structures are affected by temperature and pH changes. Such changes affect the
hrough the method of extracting the enzyme by blending it with potato tissue in phosphate buffer, the effects were analyzed on the effect of the dye guaiacol and the activity measured under different temperatures. The optimum temperature was obtained at 22.20C and above this
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