Polymerase chain reaction practical, Genetics. - Essay Example

Only on StudentShare

Extract of sample
Polymerase chain reaction practical, Genetics.

Currently, there are hundreds of different types of PCR are being used in different laboratories but the basic principle remains same. The overall process of PCR can be summarized as follows. 1) Mixture of all constituents of PCR like dNTPs, Primers (forward and reverse), Buffer and template DNA will be mixed in desired concentration. 2) The first step is amplification, where the temperature is set at 94C for 2-5 minutes for denaturation of double stranded DNA, a process called initial denaturation, 3) After initial denaturation, sample is kept for 30 sec at 94C for further denaturation, 4) After denaturation temperature is brought down to 55-60C for 30sec to allow annealing of primer with specific DNA site called annealing temperature. 5) The temperature is now brought up to the 72C for polymerase to start new DNA synthesis using primer as starting material. 6) After extension, the cycles are repeated for almost 30 times to get 230 copy of initial DNA template. Finally, after 3 cycle extension is be performed at 72C for 5 min to complete any unamplified reaction. Figure 1 shows steps involves in PCR.
Development of PCR and identification of DNA as signature molecules for individual leads to introduction of DNA based technique for establishment of parental relation and subsequently for crime and criminal detection. ...Show more

Summary

Invention of Polymerase chain reaction (PCR) by Kery Mullis in 1983(1) has revolutionized the way DNA was looked upon. PCR as name suggests is an enzyme based in vitro technique by which one can generate millions of copy of given DNA in short time…
Author : lemuelbeahan
Save Your Time for More Important Things
Let us write or edit the essay on your topic
"Polymerase chain reaction practical, Genetics."
with a personal 20% discount.
Grab the best paper

Related Essays

Genetics and Evolution
This phenomenon is clearly an evolutionary controversy. If phenotypic characters only are taken to be granted for discerning species, there really is no discerning phenotype between these species. This also means there are no clear-cut differentiating phenotypic features between the species of these geographic areas arranged in the form of a ring.
10 pages (2500 words) Essay
Polymerase Chain Reation
3 pages (750 words) Lab Report
Human Genetics
One would assume that Bob's father would be DD, with no gene for the disease. However, if his mother was Dd, and his father was DD, there is no probable chance or explanation for how Bob contracted the disease. The only possibility is that somehow Bob's father has the recessive trait and that got passed with the recessive trait from his mother to make Bob dd.
6 pages (1500 words) Essay
Polymerase Chain Reaction (PCR))
The Polymerase chain reaction was first introduced Kary Mullis in the 1980's (Bartlett et al 2003). Prior to its use in molecular biology, the amplification of DNA could only be carried out by cloning. PCR allows a "direct amplification without the use of cloning" (Bastianutto et al 2006).
2 pages (500 words) Essay
Genetics
In order for such a procedure to be done, two RAD51 targeting constructs that contained either neomycin or blasticidin first had to be prepared. Once these targeting constructs were prepared, the RAD51 neo constructs were transpected into DT40 cells, and heterozygous clones were isolated for the RAD51 gene.
3 pages (750 words) Essay
Find out how much would it cost
to get a custom paper written by a pro under your requirements!
Win a special DISCOUNT!
Put in your e-mail and click the button with your lucky finger
Your email
YOUR PRIZE:
Apply my DISCOUNT